Apoptosis in the absence of cytochrome c accumulation in the cytosol.
Apoptosis in the absence of cytochrome c accumulation in the cytosol.
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DOI：
10.1006/bbrc.1997.7969
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发表时间：
1998-01
影响因子：
3.1
通讯作者：
D. Tang;Li Li-Li;Zhenyu Zhu;B. Joshi
中科院分区：
文献类型：
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作者：
D. Tang;Li Li-Li;Zhenyu Zhu;B. Joshi
细胞色素c从线粒体释放到胞浆中并随后在胞浆中积累被认为是细胞凋亡的先决条件。在这项研究中，我们提出了在没有细胞色素c在胞浆中积累的情况下诱导细胞凋亡的证据。Staurospine处理的U937淋巴瘤细胞在PARP裂解和DNA断裂之前将细胞色素c从线粒体释放到胞浆中。然而，BMD188(一种高效的细胞凋亡诱导剂)处理的U937细胞在诱导凋亡的过程中没有发现细胞色素c在胞浆中积聚。这两种诱导剂对白血病HL60细胞和上皮性PC3细胞也有不同的细胞色素c变化。此外，当一组诱导剂处理PC3细胞时，发现喜树碱、博莱霉素、VP16和肿瘤坏死因子-α在PARP裂解之前或同时诱导不同数量的细胞色素c积聚，而长春花碱和BHPP不能。综上所述，目前的结果表明，细胞色素c在细胞凋亡期间在胞浆中的积累是一种依赖于细胞类型和诱导剂的现象。
Cytochrome c release from mitochondria to and subsequent accumulation in the cytosol has been considered a prerequisite for apoptosis. In this study, we present evidence for apoptosis induction without accumulation of cytochrome c in the cytosol. U937 lymphoma cells treated with staurosprine released cytochrome c from mitochondria to cytosol prior to PARP cleavage and DNA fragmentation. However, U937 cells treated with BMD188 (a hydroxamic acid and a potent apoptosis inducer) did not demonstrate any cytochrome c accumulation in the cytosol during apoptosis induction. This different pattern of cytochrome c alterations was also observed with these two inducers on leukemic HL60 cells and epithelial PC3 cells. Furthermore, when PC3 cells were treated with a panel of apoptosis-inducing agents, it was found that camptothecin, bleomycin, VP16 and TNF-alpha induced varying amounts of cytosolic accumulation of cytochrome c either prior to or concurrent with PARP cleavage while vinblastine and BHPP did not. Taken together, the present results suggest that cytochrome c accumulation in the cytosol during apoptosis is a cell type- and inducer-dependent phenomenon.