Advances in mass spectrometry based strategies to study receptor tyrosine kinases.
Advances in mass spectrometry based strategies to study receptor tyrosine kinases.
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DOI：
10.1107/s2052252516020546
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发表时间：
2017-03-01
期刊：
影响因子：
3.9
通讯作者：
Huang PH
中科院分区：
文献类型：
--
作者：
Vyse S;Desmond H;Huang PH
这篇综述讨论了基于质谱的策略来研究受体酪氨酸激酶信号网络和激活动力学的进展。受体酪氨酸激酶（receptor tyrosine kinases，RTK）是一种重要的跨膜环境传感器，能够将细胞外信息传递到表型反应中，包括细胞增殖、存活和代谢。基于质谱（MS）的磷酸化蛋白质组学的进展有助于为我们目前对RTK信号网络和激活动力学的理解提供基础。此外，与疾病中RTK的失调相关的新见解，例如受体共激活和激酶组重编程，已经在很大程度上使用基于磷酸化蛋白质组学的策略进行了鉴定。本文综述了目前磷酸化蛋白质组学研究的主要方法，包括磷酸肽富集和质谱数据采集方法。在这里，最近的进展，在应用MS为基础的磷酸蛋白质组学弥合关键差距，我们的知识RTK信号的重点是on.The当前的技术局限性进行了讨论和新兴领域，如计算建模，高通量磷酸蛋白质组学的工作流程和下一代单细胞的方法，以进一步了解RTK生物学的新领域突出显示。
This review discusses advances in mass spectrometry-based strategies to investigate receptor tyrosine kinase signalling networks and activation dynamics. Receptor tyrosine kinases (RTKs) are key transmembrane environmental sensors that are capable of transmitting extracellular information into phenotypic responses, including cell proliferation, survival and metabolism. Advances in mass spectrometry (MS)-based phosphoproteomics have been instrumental in providing the foundations of much of our current understanding of RTK signalling networks and activation dynamics. Furthermore, new insights relating to the deregulation of RTKs in disease, for instance receptor co-activation and kinome reprogramming, have largely been identified using phosphoproteomic-based strategies. This review outlines the current approaches employed in phosphoproteomic workflows, including phosphopeptide enrichment and MS data-acquisition methods. Here, recent advances in the application of MS-based phosphoproteomics to bridge critical gaps in our knowledge of RTK signalling are focused on. The current limitations of the technology are discussed and emerging areas such as computational modelling, high-throughput phosphoproteomic workflows and next-generation single-cell approaches to further our understanding in new areas of RTK biology are highlighted.