Dietary conjugated linoleic acid modulates phenotype and effector functions of porcine CD8+ lymphocytes
Dietary conjugated linoleic acid modulates phenotype and effector functions of porcine CD8+ lymphocytes
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DOI：
10.1093/jn/131.9.2370
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发表时间：
2001-09-01
影响因子：
4.2
通讯作者：
Wannemuehler, MJ
中科院分区：
文献类型：
--
作者：
Bassaganya-Riera, J;Hontecillas, R;Wannemuehler, MJ
体内疫苗接种和攻毒研究表明，CD 8（+）淋巴细胞对于细胞介导的抗细胞内病原体和肿瘤细胞的保护作用至关重要。外周血CD 8（+）细胞的耗竭会干扰病毒和细胞内真菌的清除、迟发型超敏反应的诱导和抗肿瘤活性。与人或小鼠相反，猪外周CD 8（+）淋巴细胞的特征在于异质表达模式（即，CD 8 α β和CD 8 α α），有助于研究次要CD 8（+）细胞亚群之间的独特特征。裂区设计中的析因（2 X 2）布置，16个区组（每组2窝）猪作为免疫处理的实验单位（即，以猪为试验对象，以大豆油和共轭亚油酸（CLA）为日粮处理单元，研究CLA对CD 8（+）细胞表型和功能的调节作用。膳食CLA补充诱导猪CD 8（+）细胞的体内扩增，包括T细胞受体（TCR）-γ δ CD 8 α α T淋巴细胞、CD 3（-）CD 16（+）CD 8 α α（猪自然杀伤细胞亚群）、TCR α β CD 8 α β T淋巴细胞和增强的特异性CD 8（+）介导的效应子功能（例如，颗粒酶活性）。外周血TCR α β CD 8 α β细胞的扩增与CD 8 α β（+）胸腺细胞百分比的增加呈正相关（r = 0.89，P < 0.01）。在功能上，CLA增强外周血淋巴细胞的细胞毒性潜力和TCR γ δ CD 8 α α细胞的增殖。总的来说，这些结果表明，饮食共轭亚油酸通过调节表型和CD 8（+）细胞的效应功能参与适应性和先天性免疫增强细胞免疫。
In vivo vaccination and challenge studies have demonstrated that CD8(+) lymphocytes are essential for the development of cell-mediated protection against intracellular pathogens and neoplasic cells. Depletion of peripheral blood CD8(+) cells interferes with clearance of viruses and intracellular fungi, induction of delayed type hypersensitivity responses and antitumoral activity. In contrast to humans or mice, porcine peripheral CD8(+) lymphocytes are characterized by a heterogeneous expression pattern (i.e., CD8 alpha beta and CD8 alpha alpha) that facilitates the study of distinctive traits among minor CD8(+) cell subsets. A factorial (2 X 2) arrangement within a split-plot design, with 16 blocks of two littermate, pigs as the experimental units for immunization treatment (i.e., unvaccinated or vaccinated with a proteinase-digested Brachyspira hyodysenteriae bacterin) and pig within block as the experimental unit for dietary treatment (soybean oil or conjugated linoleic acid) were used to investigate the phenotypic and functional regulation of CD8(+) cells by dietary conjugated linoleic acid (CLA). Dietary CLA supplementation induced in vivo expansion of porcine CD8(+) cells involving T-cell receptor (TCR)-gamma delta CD8 alpha alpha T lymphocytes, CD3(-)CD16(+)CD8 alpha alpha (a porcine natural killer cell subset), TCR alpha beta CD8 alpha beta T lymphocytes and enhanced specific CD8(+)-mediated effector functions (e.g., granzyme activity). Expansion of peripheral blood TCR alpha beta CD8 alpha beta cells was positively correlated (r = 0.89, P < 0.01) with increased percentages of CD8 alpha beta (+) thymocytes. Functionally, CLA enhanced the cytotoxic potential of peripheral blood lymphocytes and proliferation of TCR gamma delta CD8 alpha alpha cells. Collectively, these results indicate that dietary CLA enhances cellular immunity by modulating phenotype and effector functions of CD8(+) cells involved in both adaptive and innate immunity.