Identification of Photosynthesis-Associated C4 Candidate Genes through Comparative Leaf Gradient Transcriptome in Multiple Lineages of C3 and C4 Species.
Identification of Photosynthesis-Associated C4 Candidate Genes through Comparative Leaf Gradient Transcriptome in Multiple Lineages of C3 and C4 Species.
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通过比较 C3 和 C4 物种多个谱系的叶梯度转录组鉴定光合作用相关的 C4 候选基因。
DOI：
10.1371/journal.pone.0140629
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Li P
中科院分区：
文献类型：
--
作者：
Ding Z;Weissmann S;Wang M;Du B;Huang L;Wang L;Tu X;Zhong S;Myers C;Brutnell TP;Sun Q;Li P
与C3物种相比，C4作物的叶片通常具有更高的辐射、水和氮利用效率。将C4性状工程化到C3作物中已被提出作为废除生物量产量上限的最有前途的方法之一。为了更好地理解C4光合作用的功能，并筛选与C4途径相关的候选基因，对玉米、绿色狗尾草和高粱3种C4植物和水稻1种C3植物的叶片发育梯度进行了比较转录网络分析。通过基因共表达和差异共表达网络相结合的方法，我们共鉴定了128个C4特异性基因。除了经典的C4穿梭基因外，还发现了一组与光反应、淀粉和蔗糖代谢、代谢物运输以及转录调控相关的新基因。这些发现将为深入研究C3和C4物种之间的差异基因调控提供重要的信息，并为在C3作物中建立C4途径提供良好的遗传资源。
Leaves of C4 crops usually have higher radiation, water and nitrogen use efficiencies compared to the C3 species. Engineering C4 traits into C3 crops has been proposed as one of the most promising ways to repeal the biomass yield ceiling. To better understand the function of C4 photosynthesis, and to identify candidate genes that are associated with the C4 pathways, a comparative transcription network analysis was conducted on leaf developmental gradients of three C4 species including maize, green foxtail and sorghum and one C3 species, rice. By combining the methods of gene co-expression and differentially co-expression networks, we identified a total of 128 C4 specific genes. Besides the classic C4 shuttle genes, a new set of genes associated with light reaction, starch and sucrose metabolism, metabolites transportation, as well as transcription regulation, were identified as involved in C4 photosynthesis. These findings will provide important insights into the differential gene regulation between C3 and C4 species, and a good genetic resource for establishing C4 pathways in C3 crops.