Hydrolysis of soybean isoflavonoid glycosides by Dalbergia β-glucosidases
Hydrolysis of soybean isoflavonoid glycosides by Dalbergia β-glucosidases
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DOI：
10.1021/jf062885p
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发表时间：
2007-03-21
影响因子：
6.1
通讯作者：
Cairns, James R. Ketudat
中科院分区：
文献类型：
--
作者：
Chuankhayan, Phimonphan;Rimlumduan, Thipwarin;Cairns, James R. Ketudat
比较了来自豆科植物黄檀和黑黄檀的两种β-葡萄糖苷酶水解大豆中的β-葡萄糖苷的能力。D. nigrescens和D. Cochinchinensis β-葡萄糖苷酶能水解结合型大豆糖苷，而D. nigrescens β-葡糖苷酶更快地水解粗大豆提取物中的共轭和非共轭糖苷。利用高效液相色谱法测定了降香β-葡糖苷酶对共轭降香糖苷的动力学性质K-m、k（cat）和k（cat）/K-m，证实了D. nigrescens β-葡糖苷酶水解这些底物。地方nigrescens β-葡萄糖苷酶还可以有效地水解大豆粉悬浮液中的β-葡萄糖苷，表明其可用于增加豆制品中的游离β-葡萄糖苷酮。
Two beta-glucosidases from the legumes Dalbergia cochinchinensis and Dalbergia nigrescens were compared for their ability to hydrolyze isoflavonoid glycosides from soybean. Both D. nigrescens and D. cochinchinensis beta-glucosidases could hydrolyze conjugated soybean glycosides, but D. nigrescens beta-glucosidase hydrolyzed both conjugated and nonconjugated glycosides in crude soybean extract more rapidly. The kinetic properties K-m, k(cat), and k(cat)/K-m of the Dalbergia beta-glucosidases toward conjugated isoflavonoid glycosides, determined using high-performance liquid chromatography, confirmed the higher efficiency of the D. nigrescens beta-glucosidase in hydrolyzing these substrates. The D. nigrescens beta-glucosidase could also efficiently hydrolyze isoflavone glycosides in soy flour suspensions, suggesting its application to increase free isoflavones in soy products.