Induction of mucin gene expression in middle ear of rats by tumor necrosis factor-α:: Potential cause for mucoid otitis media
Induction of mucin gene expression in middle ear of rats by tumor necrosis factor-α:: Potential cause for mucoid otitis media
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DOI：
10.1086/315767
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发表时间：
2000-09-01
影响因子：
6.4
通讯作者：
Kim, Y
中科院分区：
文献类型：
--
作者：
Lin, JH;Haruta, A;Kim, Y
粘液性中耳炎（Mucoid otitis media，中耳炎）的特征在于粘蛋白浓度高的粘性流体，其积聚在中耳腔中。最近的研究表明，中耳裂的初始感染可能是中耳炎发展的关键。然而，初始感染的因素归因于刺激粘蛋白的产生还不清楚。本研究表明，肿瘤坏死因子（TNF）-α，粘液渗出液中的促炎细胞因子，显着增加Muc 2粘蛋白mRNA的表达在中耳上皮，在时间和剂量依赖性的方式。与此平行的是中耳液中粘蛋白糖蛋白的显著增加。此外，TNF-α对中耳内源性TNF-α基因的表达具有自分泌和/或旁分泌作用，这可能有助于本研究中粘蛋白的产生。这些发现表明，TNF-α通过刺激粘蛋白代谢在膀胱癌的发展中起着重要作用。
Mucoid otitis media (MOM) is characterized by viscous fluid, high in mucin concentration, which accumulates in the middle ear cavity. Recent studies suggest that initial infection in the middle ear cleft may be key to the development of MOM. However, factors of the initial infection attributed to the stimulation of mucin production are not clearly understood, This study demonstrated that tumor necrosis factor (TNF)-alpha, a proinflammatory cytokine in mucoid effusion, markedly increased Muc2 mucin mRNA expression in middle ear epithelium, in a time- and dose-dependent manner. Parallel to this was a marked increase in mucin glycoprotein in middle ear fluid. Also, TNF-alpha demonstrated an autocrine and/or paracrine effect on the expression of endogenous TNF-alpha gene in the middle ear, which may contribute to the production of mucin in this study. These findings suggest that TNF-alpha plays an important role in the development of MOM by stimulating mucin metabolism.