Collagen is reduced and disrupted in human aneurysms and dissections of ascending aorta
Collagen is reduced and disrupted in human aneurysms and dissections of ascending aorta
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DOI：
10.1016/j.humpath.2007.08.003
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发表时间：
2008-03-01
期刊：
影响因子：
3.3
通讯作者：
Gutierrez, Paulo Sampaio
中科院分区：
文献类型：
--
作者：
Borges, Luciano de Figueiredo;Jaldin, Rodrigo Gibin;Gutierrez, Paulo Sampaio
升主动脉瘤的特点是整个血管增大，而主动脉夹层 (AD) 的特点是壁在外半部分分裂成两片。我们研究了胶原蛋白的变化是否与这些疾病有关。对 14 例急性夹层患者、10 例动脉瘤患者和 9 例对照受试者的主动脉切片进行天狼星染色。在偏光显微镜下分析载玻片以评估胶原纤维的结构。通过计算机图像分析系统中的颜色检测来计算内侧层每一半中胶原蛋白的比例。偏振光下的胶原外观与胶原溶解一致。对照组内半部和外半部的平均胶原比例分别为0.50 +/- 0.13和0.40 +/- 0.08，AD组为0.20 +/- 0.10和0.18 +/- 0.12，动脉瘤组为0.33 +/- 0.12和0.19 +/- 0.12。 AD (P < .01) 和对照组 (P = .04) 外半部胶原蛋白较少，动脉瘤组没有发现差异 (P = .71)。在两半中，病例患者的胶原蛋白含量均低于对照组（均 P < .01），但在内半部分，动脉瘤病例患者的胶原蛋白减少量显着大于夹层患者（P = .03；外半部分，P = .99）。主动脉夹层和动脉瘤显示胶原蛋白含量减少，这可能与疾病背后的壁薄弱有关，但减少的位置不同：在夹层中，胶原蛋白含量主要位于中层的外部部分（乳沟部位），而在动脉瘤中，胶原蛋白含量更加弥散，与整体扩大一致。 (c) 2008 Elsevier Inc. 保留所有权利。
In ascending aorta aneurysms, there is an enlargement of the whole vessel, whereas aortic dissections (ADs) are characterized by the cleavage of the wall into 2 sheets at the external half. We searched if alterations in collagen could be related to these diseases. Sections of aortas from 14 case patients with acute dissections, 10 case patients with aneurysms, and 9 control subjects were stained with picrosirius. Slides were analyzed under polarized microscopy to evaluate the structure of collagen fibers. The proportion of collagen was calculated in each half of the medial layer by color detection in a computerized image analysis system. Collagen appearance under polarized light was consistent with collagenolysis. The mean collagen proportions at the inner and outer halves, respectively, were 0.50 +/- 0.13 and 0.40 +/- 0.08 in the control group, 0.20 +/- 0.10 and 0.18 +/- 0.12 in the AD group, and 0.33 +/- 0.12 and 0.19 +/- 0.12 in the aneurysm group. The AD (P < .01) and control (P = .04) groups had less collagen at the external half, no difference was found in the aneurysm group (P = .71). In both halves, there was less collagen in the case patients than in the control subjects (all P < .01), but at the internal half, the decrease was significantly greater in the case patients with aneurysms than in those with dissections (P = .03; at the external half, P = .99). Aortic dissections and aneurysms show a decrease in collagen content that could be related to a weakness of the wall underlying the diseases, but the locations of the decrease differ: in dissections, it is situated mostly at the external portion of the media (site of cleavage), whereas in aneurysms, it is more diffuse, consistent with the global enlargement. (c) 2008 Elsevier Inc. All rights reserved.