The lymphoid past of mouse plasmacytoid cells and thymic dendritic cells
The lymphoid past of mouse plasmacytoid cells and thymic dendritic cells
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DOI：
10.4049/jimmunol.170.10.4926
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发表时间：
2003-05-15
影响因子：
4.4
通讯作者：
Shortman, K
中科院分区：
文献类型：
--
作者：
Corcoran, L;Ferrero, I;Shortman, K
关于某些树突状细胞（DC）亚型可能的淋巴起源一直存在争议。为了解决这一问题，我们分析了从正常小鼠组织中分离的DC和浆细胞样前DC的早期淋巴细胞发育的瞬时（mRNA）和永久（DNA重排）标记。来自胸腺的CD8(+) DC约27%的DNA和来自脾脏和胸腺的浆细胞样DC的22-35%的DNA含有IgH基因D-J重排，而T细胞的这一比例为40%。然而，DC DNA不包含IgH基因V-D-J重排，也不包含T细胞Ag受体13基因D-J重排。含有IgH D-J重排的相同DC谱系群体表达CD3链和pre-Talpha的mRNA。相比之下，来自脾脏、淋巴结或皮肤的常规DC的DNA，无论是CD8（+）还是CD8(-)，都很少含有IgH D-J重排，脾常规DC表达的CD3epsilon或pre-Ta mRNA非常少。因此，许多浆细胞样前DC和胸腺CD8(+) DC与淋巴细胞谱系有共同的早期发展步骤，与传统的外周DC的起源不同。
There has been controversy over the possible lymphoid origin of certain dendritic cell (DC) subtypes. To resolve this issue, DC and plasmacytoid pre-DC isolated from normal mouse tissues were analyzed for transient (mRNA) and permanent (DNA rearrangement) markers of early stages of lymphoid development. About 27% of the DNA of CD8(+) DC from thymus, and 22-35% of the DNA of plasmacytoid pre-DC from spleen and thymus, was found to contain IgH gene D-J rearrangements, compared with 40% for T cells. However, the DC DNA did not contain IgH gene V-D-J rearrangements nor T cell Ag receptor 13 gene D-J rearrangements. The same DC lineage populations containing IgH D-J rearrangements expressed mRNA for CD3 chains, and for pre-Talpha. In contrast, little of the DNA of the conventional DC derived from spleen, lymph nodes, or skin, whether CD8(+) or CD8(-), contained IgH D-J rearrangements and splenic conventional DC expressed very little CD3epsilon or pre-Ta mRNA. Therefore, many plasmacytoid pre-DC and thymic CD8(+) DC have shared early steps of development with the lymphoid lineages, and differ in origin from conventional peripheral DC.