Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis
Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis
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DOI：
10.1128/aem.68.8.3919-3924.2002
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发表时间：
2002-08-01
影响因子：
4.4
通讯作者：
Lee, JK
中科院分区：
文献类型：
--
作者：
Lee, SJ;Park, SY;Lee, JK
革兰氏阴性菌之间可以通过N-酰基高丝氨酸内酯（AHLs）进行通讯，AHLs是一种群体感应自诱导物。最近，aiiA基因（编码催化AHL降解的酶）已从芽孢杆菌属菌株240 B1中克隆。在正在进行的苏云金芽孢杆菌亚种的调查过程中，morrisoni基因组计划中发现了一个aiiA同源基因，这些结果使人们考虑到该基因在B中广泛存在的可能性。苏云金杆菌在B的16个亚种中发现了aiiA同源基因。苏云金杆菌（thuringiensis），并测定了它们的序列。芽孢杆菌属菌株240 B1 aiiA基因与B.苏云金杆菌aiiA同源基因的核苷酸序列和推导的氨基酸序列同源性分别为89%~95%和90%~96%。在B的亚种中。在具有aiiA基因的苏云金杆菌中，亚种aizawai、galleriae、kurstaki、kyushuensis、ostriniae和subtoxicus显示降解AHL。结果表明，表达AiiA蛋白的重组大肠杆菌也具有AHL降解活性，并能减弱胡萝卜软腐欧文氏菌对植物的致病性。这些结果表明，杀虫B。苏云金杆菌菌株可能具有通过自身诱导物降解活性与自然生态系统中的革兰氏阴性菌竞争的潜力。
Gram-negative bacteria can communicate with each other by N-acyl homoserine lactones (AHLs), which are quorum-sensing autoinducers. Recently, the aiiA gene (encoding an enzyme catalyzing the degradation of AHL) has been cloned from Bacillus sp. strain 240B1. During investigations in the course of the ongoing Bacillus thuringiensis subsp. morrisoni genome project, an aiiA homologue gene in the genome sequence was found. These results led to consideration of the possibility of the widespread existence of the gene in B. thuringiensis. aiiA homologue genes were found in 16 subspecies of B. thuringiensis, and their sequences were determined. Comparison of the Bacillus sp. strain 240B1 aiiA gene with the B. thuringiensis aiiA homologue genes showed high homologies of 89 to 95% and 90 to 96% in the nucleotide sequence and deduced amino acid sequence, respectively. Among the subspecies of B. thuringiensis having an aiiA gene, the subspecies aizawai, galleriae, kurstaki, kyushuensis, ostriniae, and subtoxicus were shown to degrade AHL. It was observed that recombinant Escherichia coli producing AiiA proteins also had AHL-degrading activity and could also attenuate the plant pathogenicity of Erwinia carotovora. These results indicate that insecticidal B. thuringiensis strains might have potential to compete with gram-negative bacteria in natural ecosystems by autoinducer-degrading activity.