Capsaicin-induced transcriptional changes in hypothalamus and alterations in gut microbial count in high fat diet fed mice
Capsaicin-induced transcriptional changes in hypothalamus and alterations in gut microbial count in high fat diet fed mice
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DOI：
10.1016/j.jnutbio.2014.04.004
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发表时间：
2014-09-01
影响因子：
5.6
通讯作者：
Bishnoi, Mahendra
中科院分区：
文献类型：
--
作者：
Baboota, Ritesh K.;Murtaza, Nida;Bishnoi, Mahendra
肥胖是一个全球性的健康问题，最近它已被视为发展中国家日益关注的问题。几种生物活性膳食分子与肥胖和相关并发症的改善有关，辣椒素是其中之一。本研究试图了解辣椒素在高脂饮食（HFD）喂养小鼠中抗肥胖活性的新机制并为其提供证据。将瑞士白化病小鼠分为三组（n=8-10），即对照组、喂食HFD组和喂食辣椒素（2 mg/kg，po）+HFD组，分别给予3个月的治疗。在测量表型和血清相关的生化变化后，研究并定量辣椒素对下丘脑、白色脂肪组织（WAT）（内脏和皮下）、棕色脂肪组织（BAT）和肠道微生物改变中HFD诱导的转录变化的影响。我们的研究结果表明，除了其众所周知的影响，口服辣椒素（a）调节下丘脑饱腹感相关的基因型，（B）改变肠道微生物组成，（c）诱导“布朗宁”基因型（BAT相关基因）在皮下WAT和（d）增加的产热和线粒体生物合成基因的表达BAT。本研究为辣椒素的抗肥胖作用提供了新的和有趣的机制。(C)2014爱思唯尔公司All rights reserved.
Obesity is a global health problem and recently it has been seen as a growing concern for developing countries. Several bioactive dietary molecules have been associated with amelioration of obesity and associated complications and capsaicin is one among them. The present work is an attempt to understand and provide evidence for the novel mechanisms of anti-obesity activity of capsaicin in high fat diet (HFD)-fed mice. Swiss albino mice divided in three groups (n=8-10) i.e. control, HFD fed and capsaicin (2 mg/kg, po)+HFD fed were administered respective treatment for 3 months. After measuring phenotypic and serum related biochemical changes, effect of capsaicin on HFD-induced transcriptional changes in hypothalamus, white adipose tissue (WAT) (visceral and subcutaneous), brown adipose tissue (BAT) and gut microbial alterations was studied and quantified. Our results suggest that, in addition to its well-known effects, oral administration of capsaicin (a) modulates hypothalamic satiety associated genotype, (b) alters gut microbial composition, (c) induces "browning" genotype (BAT associated genes) in subcutaneous WAT and (d) increases expression of thermogenesis and mitochondrial biogenesis genes in BAT. The present study provides evidence for novel and interesting mechanisms to explain the anti-obesity effect of capsaicin. (C) 2014 Elsevier Inc. All rights reserved.