Toll-like receptor 2 of tongue sole Cynoglossus semilaevis: Signaling pathway and involvement in bacterial infection
Toll-like receptor 2 of tongue sole Cynoglossus semilaevis: Signaling pathway and involvement in bacterial infection
复制标题
半滑舌鳎Toll样受体2：信号通路及其参与细菌感染
DOI：
10.1016/j.fsi.2016.03.001
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发表时间：
2016-04-01
影响因子：
4.7
通讯作者：
Sun, Li
中科院分区：
文献类型：
--
作者：
Li, Xue-peng;Sun, Li
Toll样受体（TLR）2是TLR家族的成员，在先天免疫中起关键作用。在哺乳动物中，已知TLR 2识别特定的微生物结构并触发MyD 88依赖性信号传导以诱导各种细胞因子应答。本研究检测了半滑舌鳎TLR 2、CsTLR 2的表达和功能。CsTLR 2由898个氨基酸残基组成，与已知的硬骨鱼类TLR 2共有25.6%-273%的总序列同一性。CsTLR 2是一种跨膜蛋白，具有Toll/白细胞介素-1受体结构域和八个富含亮氨酸的重复序列。CsTLR 2在多种组织中表达，并在细菌感染过程中上调。CsTLR 2通路的刺激导致MyD 88依赖性信号分子的表达增强。重组CsTLR 2（rCsTLR 2）对应于胞外区，能够与广泛的细菌结合。在体外和体内条件下，rCsTLR 2显着减少细菌感染。这些观察为硬骨鱼TLR 2的信号传导和功能提供了新的见解。(C)2016爱思唯尔有限公司版权所有
Toll-like receptor (TLR) 2 is a member of the TLR family that plays a pivotal role in innate immunity. In mammals, TLR2 is known to recognize specific microbial structures and trigger MyD88-dependent signaling to induce various cytokine responses. In this study, we examined the expression and function of the tongue sole Cynoglossus semilaevis TLR2, CsTLR2. CsTLR2 is composed of 898 amino acid residues and shares 25.6%-273% overall sequence identities with known teleost TLR2. CsTLR2 is a transmembrane protein with a toll/interleukin-1 receptor domain and eight leucine-rich repeats. Expression of CsTLR2 occurred in multiple tissues and was upregulated during bacterial infection. Stimulation of the CsTLR2 pathway led to enhanced expression of MyD88-dependent signaling molecules. Recombinant CsTLR2 (rCsTLR2) corresponding to the extracellular region was able to bind to a wide range of bacteria. Under both in vitro and in vivo conditions, rCsTLR2 significantly reduced bacterial infection. These observations add new insights into the signaling and function of teleost TLR2. (C) 2016 Elsevier Ltd. All rights reserved.