Involvement of caspase activation through release of cytochrome c from mitochondria in apoptotic cell death of macrophages infected with Actinobacillus actinomycetemcomitans.
Involvement of caspase activation through release of cytochrome c from mitochondria in apoptotic cell death of macrophages infected with Actinobacillus actinomycetemcomitans.
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DOI：
10.2504/kds.58.60_2
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发表时间：
2004-04
影响因子：
2.1
通讯作者：
Hironori Kasai;Kozo Yamamoto;T. Koseki;M. Yokota;T. Nishihara
中科院分区：
文献类型：
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作者：
Hironori Kasai;Kozo Yamamoto;T. Koseki;M. Yokota;T. Nishihara
我们以前报道过，感染牙周病细菌放线杆菌放线菌伴放线杆菌诱导小鼠巨噬细胞系J774.1的凋亡。在本研究中，我们检测了细胞色素c和半胱天冬酶在A.放线菌共生体感染的J774.1细胞。感染后，使用免疫印迹分析检测细胞中细胞色素c的表达水平以及半胱天冬酶-3和半胱天冬酶-9的裂解形式。细胞色素c在A.共放线菌感染的J774.1细胞培养6小时，发现caspase-3和caspase-9在细胞中是裂解形式。此外，caspase-9的活性显着增加，磷酸化的p53在感染后30小时的细胞中检测到。这些结果提示，A.放线菌共生体感染的J774.1细胞通过细胞色素c从线粒体释放到细胞质中以及随后通过p53磷酸化激活半胱天冬酶来调节。
We previously reported that infection with the periodontopathic bacterium Actinobacillus actinomycetemcomitans induced apoptosis in a mouse macrophage cell line J774.1. In the present study, we examined the involvement of cytochrome c and caspases in the induction of apoptosis in A. actinomycetemcomitans-infected J774.1 cells. Following infection, the expression levels of cytochrome c, and cleaved forms of caspase-3 and caspase-9 in the cells were examined using immunoblot analysis. Cytochrome c was released from mitochondria into the cytoplasm after A. actinomycetemcomitans-infected J774.1 cells were cultured for 6 h, and caspase-3 and caspase-9 were found to be cleaved forms in the cells. Further, caspase-9 activity was markedly increased, and phosphorylated p53 was detected in the cells 30 h following infection. These results suggest that apoptosis in A. actinomycetemcomitans-infected J774.1 cells is regulated by the release of cytochrome c from mitochondria into cytoplasm and the subsequent activation of caspases through phosphorylation of p53.