Gas Exchange Measurements for the Determination of Photosynthetic Efficiency in Arabidopsis Leaves
Gas Exchange Measurements for the Determination of Photosynthetic Efficiency in Arabidopsis Leaves
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DOI：
10.1007/978-1-61779-237-3_17
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Murchie, Erik
中科院分区：
文献类型：
--
作者：
Johnson, Giles;Murchie, Erik
光合作用是植物中最容易测量的代谢过程之一，即使在野外条件下，其通量也可以无创测量。在本章中，描述了测量光合作用的两种主要方法-使用O-2电极确定O-2的演变，以及可以使用红外气体分析仪量化的CO2固定。本文讨论了这些不同方法在拟南芥研究中的优缺点，并介绍了几种主要的分析方法。
Photosynthesis is one of the most readily measured metabolic processes in a plant, with fluxes being measurable non-invasively even under field conditions. In this chapter, two principal approaches are described to measure photosynthesis - O-2 evolution as determined using an O-2 electrode, and CO2 fixation which can be quantified using an infrared gas analyser. The advantages and disadvantages of these different methods, as applied to Arabidopsis, arc discussed, and some major forms of analysis are described.