Biophysical Characterization of Flagellar Motor Functions
Biophysical Characterization of Flagellar Motor Functions
复制标题
DOI：
10.3791/55240
复制
发表时间：
2017-01-01
影响因子：
1.2
通讯作者：
Lele, Pushkar P.
中科院分区：
文献类型：
--
作者：
Ford, Katie M.;Chawla, Ravi;Lele, Pushkar P.
鞭毛马达在细菌运动和趋化中的作用是众所周知的。最近的发现表明，鞭毛马达能够对各种环境刺激做出重塑，并是表面定植和感染的触发因素之一。马达重塑和促进细胞适应的确切机制可能取决于关键的马达属性。基于光电倍增管的磁珠跟踪技术使运动功能的准确生物物理表征成为可能，包括对电机速度和开关动力学的适应。这种方法提供了实时跟踪的优势，并能够在更长的持续时间内探测电机行为。所讨论的方案可以很容易地扩展到研究各种细菌物种的鞭毛马达。
The role of flagellar motors in bacterial motility and chemotaxis is well-understood. Recent discoveries suggest that flagellar motors are able to remodel in response to a variety of environmental stimuli and are among the triggers for surface colonization and infections. The precise mechanisms by which motors remodel and promote cellular adaptation likely depend on key motor attributes. The photomultiplier-based bead-tracking technique presented here enables accurate biophysical characterization of motor functions, including adaptations in motor speeds and switch-dynamics. This approach offers the advantage of real-time tracking and the ability to probe motor behavior over extended durations. The protocols discussed can be readily extended to study flagellar motors in a variety of bacterial species.