The correlation of protective effects and antibody production in immunized chickens with recombinant R7 vaccine against Leucocytozoon caulleryi
The correlation of protective effects and antibody production in immunized chickens with recombinant R7 vaccine against Leucocytozoon caulleryi
复制标题
DOI：
10.1292/jvms.64.405
复制
发表时间：
2002-05-01
影响因子：
1.2
通讯作者：
Gotanda, T
中科院分区：
文献类型：
--
作者：
Itoh, A;Gotanda, T
研究了卡氏住白细胞虫重组R7疫苗对鸡的免疫保护作用。将油佐剂rR 7疫苗注射鸡后，抗乳杆菌第二代裂殖体（2GS）抗原的抗体滴度。Calleryi（抗2GS抗体）在所有免疫鸡中迅速升高，在注射后2周达到峰值，并且滴度持续到注射后4或5个月。在攻击前具有高水平的抗2GS抗体滴度（大于或等于102，400）的鸡完全保护免受卡氏利什曼原虫子孢子攻击。攻毒接种后。相对较高的寄生虫血症。在攻击前具有低水平抗体滴度（小于或等于3，200）的所有未充分免疫的鸡中观察到Caulleryi。虽然有些人看起来很正常免疫鸡的保护效果与攻虫后临床症状和虫血症的预防和攻虫前抗2GS抗体滴度之间存在相关性。本研究表明，鸡白细胞体病可以通过免疫接种来预防，体液免疫在控制鸡白细胞体病中可能起重要作用。
Protective effects of recombinant R7 (rR7) vaccine against Leucocytozoon caulleryi in chickens were studied. After injection of oil-adjuvanted rR7 vaccine into chickens, antibody titers against second-generation schizonts (2GS) antigen of L. caulleryi (anti-2GS antibody) rapidly rose in all the immunized chickens, reached to a peak value 2 weeks after injection, and the titers persisted through 4 or 5 months after injection. Chickens having high levels of the anti-2GS antibody titers (greater than or equal to 102,400) at pre-challenge completely protected against sporozoites challenge of L caulleryi. After the challenge inoculation. relatively high parasitemia of L. caulleryi was observed in all the inadequately immunized chickens having low levels of the antibody titers (less than or equal to 3,200) at pre-challenge. although some of them seemed to be clinically normal. Correlation of protective effects in the immunized chickens was observed between both prevention of appearance of clinical signs and parasitemia after parasites challenge and anti-2GS antibody titers of the chickens at pre-challenge. The present study shows that chicken leucocytozoonosis can be prevented by vaccination, and humoral immunity may play an important role in the control of chicken leucocytozoonosis.