Electron microscopy of chromatin.
Electron microscopy of chromatin.
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染色质电子显微镜。
DOI：
10.1006/meth.1997.0450
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Horowitz,RA
中科院分区：
文献类型：
--
作者：
Woodcock,CL;Horowitz,RA
电子显微镜能够对 DNA 和核小体进行成像，可以为理解染色质构象提供关键的视觉联系。我们讨论 EM 在当前染色质研究中的应用，重点讨论消除与传统 EM 制备技术相关的许多潜在问题的策略。详细考虑了分离染色质的冷冻电子显微镜 (cryo-EM)，即样品在玻璃化薄膜中的“溶液中”成像，重点是三维信息的恢复及其在连接 DNA 构象和盐诱导压缩中的应用。还考虑了目前限制该技术的因素以及克服这些因素的前景。
Electron microscopy, with its ability to image DNA and nucleosomes, can provide a key visual link in the understanding of chromatin conformation. We discuss applications of EM to current chromatin research with emphasis on strategies that eliminate many of the potential problems associated with conventional EM preparative techniques. Cryo-electron microscopy (cryo-EM) of isolated chromatin, whereby samples are imaged “in solution” in thin vitrified films, is considered in detail, with emphasis on the recovery of three-dimensional information and on its application to linker DNA conformation and to salt-induced compaction. Factors that currently limit the technique, and the prospects of overcoming them, are also considered.