Chlamydomonas starchless mutant defective in ADP-glucose pyrophosphorylase hyper-accumulates triacylglycerol
Chlamydomonas starchless mutant defective in ADP-glucose pyrophosphorylase hyper-accumulates triacylglycerol
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DOI：
10.1016/j.ymben.2010.02.002
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发表时间：
2010-07-01
影响因子：
8.4
通讯作者：
Hua, Qiang
中科院分区：
文献类型：
--
作者：
Li, Yantao;Han, Danxiang;Hua, Qiang
许多微藻和植物都有能力合成大量的三酰甘油（TAG），可用于生产生物燃料。目前，基于TAG的生物燃料生产受到原料供应的限制。在产油微藻和油料作物植物中过量生产TAG的脂质合成途径的代谢工程仅取得了适度的成功。我们证明，ADP-葡萄糖焦磷酸化酶在衣原体strachless突变体的失活导致TAG增加了10倍，这表明从淀粉到TAG合成的光合碳分配的分流可能是一种比直接操纵脂质合成途径过度生产TAG更有效的策略。(C)2010年爱思唯尔公司All rights reserved.
Many microalgae and plants have the ability to synthesize large amounts of triacylglycerol (TAG) that can be used to produce biofuels. Presently, TAG-based biofuel production is limited by the feedstock supply. Metabolic engineering of lipid synthesis pathways to overproduce TAGs in oleaginous microalgae and oil crop plants has achieved only modest success. We demonstrate that inactivation of ADP-glucose pyrophosphorylase in a Chlamydomonas strachless mutant led to a 10-fold increase in TAG, suggesting that shunting of photosynthetic carbon partitioning from starch to TAG synthesis may represent a more effective strategy than direct manipulation of the lipid synthesis pathway to overproduce TAG. (C) 2010 Elsevier Inc. All rights reserved.