Identification of chilling-responsive microRNAs and their targets in vegetable soybean (Glycine max L.).
Identification of chilling-responsive microRNAs and their targets in vegetable soybean (Glycine max L.).
复制标题
菜用大豆 (Glycine max L.) 中寒冷响应性 microRNA 及其靶标的鉴定
DOI：
10.1038/srep26619
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发表时间：
2016-05-24
影响因子：
4.6
通讯作者：
Gong Y
中科院分区：
文献类型：
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作者：
Xu S;Liu N;Mao W;Hu Q;Wang G;Gong Y
低温胁迫是限制菜用大豆（Glycine max L.）在全球范围内。本研究对低温胁迫下miRNAs进行了系统的鉴定和功能分析，旨在阐明小麦抗冷性的分子机制。构建了两个独立的大豆叶片小RNA文库，并利用高通量Illumina Solexa系统进行了测序。共鉴定出434个已知的miRNAs和3个新的miRNAs。通过qRT-PCR分析验证了35个miRNA。此外，通过高通量degradome测序鉴定其基因靶点。共有898个转录本被归于五类的54个miRNA家族靶向。更重要的是，我们发现了51个在低温胁迫和对照条件下差异表达的miRNAs。这些miRNAs的作用靶点主要集中在氧化还原、信号转导和代谢过程等功能类别。我们的qRT-PCR分析证实了在低温胁迫下miRNAs与其靶基因之间的负相关性。因此，我们的工作提供了全面的分子证据，支持参与菜用大豆的冷胁迫反应的miRNAs。
Chilling stress is a major factor limiting the yield and quality of vegetable soybean (Glycine max L.) on a global scale. In the present study, systematic identification and functional analysis of miRNAs under chilling stress were carried out to clarify the molecular mechanism of chilling resistance. Two independent small RNA libraries from leaves of soybean were constructed and sequenced with the high-throughput Illumina Solexa system. A total of 434 known miRNAs and 3 novel miRNAs were identified. Thirty-five miRNAs were verified by qRT-PCR analysis. Furthermore, their gene targets were identified via high-throughput degradome sequencing. A total of 898 transcripts were targeted by 54 miRNA families attributed to five categories. More importantly, we identified 51 miRNAs differentially expressed between chilling stress and control conditions. The targets of these miRNAs were enriched in oxidation-reduction, signal transduction and metabolic process functional categories. Our qRT-PCR analysis confirmed a negative relationship among the miRNAs and their targets under chilling stress. Our work thus provides comprehensive molecular evidence supporting the involvement of miRNAs in chilling-stress responses in vegetable soybean.