Effects of Calcium on Photosynthesis, Antioxidant System, and Chloroplast Ultrastructure in Tomato Leaves Under Low Night Temperature Stress
Effects of Calcium on Photosynthesis, Antioxidant System, and Chloroplast Ultrastructure in Tomato Leaves Under Low Night Temperature Stress
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DOI：
10.1007/s00344-014-9462-9
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发表时间：
2015-06
影响因子：
4.8
通讯作者：
Yu-feng Liu;Guoxian Zhang;M. Qi;Tianlai Li
中科院分区：
文献类型：
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作者：
Yu-feng Liu;Guoxian Zhang;M. Qi;Tianlai Li
研究了低温条件下外源cacl2对番茄叶片光合作用、抗氧化系统和叶绿体超微结构的影响。番茄幼苗分别用27 mM CaCl2和5 mM EGTA预处理4 d，然后在6℃条件下用LNT处理7 d。与蒸馏水处理相比，cacl2处理的植株在LNT胁迫下光合作用增强，气孔开度增大，叶绿体面积增大，淀粉粒数减少。LNT增加了超氧自由基、过氧化氢和丙二醛的含量，而施用钙则降低了这些化合物的含量。外源钙的施用也增强了LNT胁迫引起的抗氧化活性的增加。乙二醇-双-（2-氨基乙基）四乙酸预处理效果相反。施用钙提高了光照胁迫下植物的光合作用。这种改善与气孔导度增加、抗氧化活性提高和叶绿体结构调整有关。这些结果表明，施用cac2改善了光照胁迫下植物的光合作用。为进一步研究分子机理提供了基础资料。
The effect of exogenous CaCl2on photosynthesis, antioxidant system, and chloroplast ultrastructure of tomato leaves under low night temperature (LNT) was investigated. Tomato seedlings were pretreated with 27 mM CaCl2, 5 mM EGTA, respectively, for 4 days and then exposed to LNT treatment at 6 °C for 7 days. Plants treated with CaCl2showed higher photosynthesis, increased stomatal aperture and chloroplast area, and decreased number of starch grains under LNT stress than those treated with distilled water. LNT increased the contents of superoxide radicals, hydrogen peroxide, and malondialdehyde, whereas calcium application reduced the contents of these compounds. The increase in antioxidant activities caused by LNT stress was also enhanced by the application of exogenous calcium. The opposite effects were observed with ethylene glycol-bis-(2-aminoethyl) tetraacetic acid pretreatment. CaCl2application improved photosynthesis in LNT-stressed plants. Such improvement was associated with increased stomatal conductance, improved antioxidant activities, and adjusted chloroplast structure. These results suggest that CaCl2application improved photosynthesis in LNT-stressed plants. It provided fundamental information for further molecular mechanism research.