A single naive CD8+ T cell precursor can develop into diverse effector and memory subsets
A single naive CD8+ T cell precursor can develop into diverse effector and memory subsets
复制标题
DOI：
10.1016/j.immuni.2007.10.012
复制
发表时间：
2007-12-01
期刊：
影响因子：
32.4
通讯作者：
Busch, Dirk H.
中科院分区：
文献类型：
--
作者：
Stemberger, Christian;Huster, Katharina M.;Busch, Dirk H.
在第一次遇到抗原时，初始CD8（+）T细胞被激活，克隆扩增，并可以发育成非常不同的亚群，如短寿命效应细胞或不同的记忆亚群。亚群多样化的起源目前尚不清楚，但单个前体细胞接收的早期信号的定性和定量差异已被认为是一个主要的决定因素。我们表明，一个单一的抗原特异性幼稚T细胞转移到一个正常的受体小鼠允许克隆扩增的子细胞免疫后恢复。通过这种实验方法，我们最终证明了广泛的多样性可以从单个前体细胞中发展出来，包括不同类型的效应和记忆T细胞。有趣的是，单细胞衍生的亚群多样化类似于同一个小鼠中的多克隆T细胞反应，尽管免疫策略之间的分化模式不同。这些数据暗示，子集多样化的形状和同步在扩展阶段。
Upon first antigen encounter, naive CD8(+) T cells get activated, clonally expand, and can develop into very distinct subsets, such as short-living effector cells or different memory subpopulations. The origin of subset diversification is currently unknown, but qualitative and quantitative differences in early signals received by individual precursor cells have been suggested as a major determinant. We show that transfer of a single antigen-specific naive T cell into a normal recipient mouse allowed recovery of clonally expanded daughter cells upon immunization. With this experimental approach, we conclusively demonstrated that a wide range of diversity could develop out of a single precursor cell, including different types of effector and memory T cells. Interestingly, single-cell-derived subset diversification resembled that of polyclonal T cell responses in the same individual mouse, although differentiation patterns differed between immunization strategies. These data implicate that subset diversification is both shaped and synchronized during the expansion phase.