Changes in caecal microbiota and mucosal morphology of weaned pigs
Changes in caecal microbiota and mucosal morphology of weaned pigs
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DOI：
10.1016/j.vetmic.2007.04.026
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发表时间：
2007-10-06
影响因子：
3.3
通讯作者：
Gasa, Josep
中科院分区：
文献类型：
--
作者：
Castillo, Marisol;Martin-Orue, Susana M.;Gasa, Josep
设计了一个实验来监测盲肠微生物群的变化。与早期断奶有关。十二仔猪（20 - 2天）从6个不同的窝从商业来源选择。对于两个实验组，将每窝中的一只动物断奶至断奶后饮食（W），另一只动物与母猪（S）保持在一起。1周后，处死动物并采集盲肠样品。使用SYBR绿色（R）染料通过定量PCR测定总细菌、肠杆菌和乳酸杆菌种群的微生物计数。通过末端限制性片段长度多态性（t-RFLP）评估微生物概况。断奶促进了肠杆菌与雅氏杆菌比率的增加（0.27对1.67 log/log 16 S rRNA基因拷贝数，P = 0.05）。盲肠微生物生态系统的总细菌和丰富度（峰数）在两个实验组中相似（S组为49.3，W组为53.4，P = 0.22），尽管通过树状图分析，条带模式明显分为两个不同的簇。断乳还与隐窝密度降低、肌萎缩指数增加和杯状细胞数量减少有关。还观察到免疫反应降低，表现为固有层上皮内淋巴细胞和淋巴细胞密度增加。断奶似乎对建立猪盲肠微生物群至关重要，尤其是微生物群和盲肠粘膜结构发生了重要变化。(c)2007 Elsevier B. V.保留所有权利。
An experiment was designed to monitor the changes in caecal microbiota. associated with early weaning. Twelve piglets (20 2 days) from six different litters were selected from a commercial source. For the two experimental groups, one animal from each litter was weaned onto a post-weaning diet (W) and the other remained with the sow (S). After 1 week, animals were sacrificed and caecal samples taken. Microbial counts for total bacteria, enterobacteria and lactobacilli populations were determined by quantitative PCR using SYBR Green (R) dye. Microbial profiles were assessed by terminal restriction fragment length polymorphism (t-RFLP). Weaning promoted an increase in the enterobacteriaJactobacilli ratio (0.27 versus 1.67 log/log 16S rRNA gene copy number, P = 0.05). Total bacteria and richness of the caecal microbial ecosystem (number of peaks) were similar in both experimental groups (49.3 for S and 53.4 for W, respectively, P = 0.22), although the band patterns were clearly grouped in two different clusters by dendogram analysis. Weaning was also associated with a decrease in crypt density, an increase in mytotic index and a decrease in the number of goblet cells. A reduced immunological response was also observed and was manifested by an increase in intraepithelial lymphocytes and lymphocyte density in the lamina propria. Weaning appears to be critical in the establishment of the caecal microbiota in pigs with important changes, particularly in microbial groups and in caecal mucosal architecture. (c) 2007 Elsevier B.V. All rights reserved.