Toxic effect of blood components on perinatal rat subventricular zone cells and oligodendrocyte precursor cell proliferation, differentiation and migration in culture
Toxic effect of blood components on perinatal rat subventricular zone cells and oligodendrocyte precursor cell proliferation, differentiation and migration in culture
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DOI：
10.1111/j.1471-4159.2009.06060.x
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发表时间：
2009-06-01
影响因子：
4.7
通讯作者：
Del Bigio, Marc R.
中科院分区：
文献类型：
--
作者：
Juliet, Packiasamy A. R.;Frost, Emma E.;Del Bigio, Marc R.
人的大脑中胚基质在妊娠中期后产生少突胶质细胞和星形胶质细胞。早产儿生殖基质出血与细胞增殖抑制有关。我们假设可溶性血液成分对培养的大鼠脑室下区（SVZ）细胞的增殖和少突胶质祖细胞（OPC）的增殖、迁移和分化有不利影响。使用半胱天冬酶3激活和乳酸脱氢酶释放测定，大鼠血浆，血清，凝血酶，和激肽释放酶杀死SVZ细胞时，生长在血小板衍生的生长因子的存在下（但不是不存在）。血浆和血清以1：1至1：100稀释度杀死OPC。使用溴脱氧尿苷掺入试验，OPC增殖减少血浆，血清，凝血酶和纤溶酶。血蛋白还以浓度依赖性方式抑制OPC迁移。然而，分化成髓鞘碱性蛋白表达细胞的OPC被抑制，只有凝血酶。我们的结论是，可溶性血液成分，特别是凝血酶，有一个成熟的SVZ细胞和OPC来自新生大鼠脑的不利影响。
The germinal matrix of human brain gives rise to oligodendrocytes and astrocytes after mid-gestation. Hemorrhage in the germinal matrix of premature infants is associated with suppressed cell proliferation. We hypothesize that soluble blood constituents have an adverse effect on the proliferation of cultured rat subventricular zone (SVZ) cells and the proliferation, migration, and differentiation of oligodendrocyte progenitor cells (OPC). Using caspase 3 activation and lactate dehydrogenase release assays, rat plasma, serum, thrombin, and kallikrein killed SVZ cells when grown in the presence (but not absence) of platelet derived growth factor. Plasma and serum killed OPC at 1 : 1 to 1 : 100 dilutions. Using a bromodeoxyuridine incorporation assay OPC proliferation was reduced by plasma, serum, thrombin and plasmin. Blood proteins also suppressed OPC migration in a concentration dependent manner. However, differentiation of OPC into myelin basic protein expressing cells was suppressed only by thrombin. We conclude that soluble blood components, particularly thrombin, have an adverse effect on maturing SVZ cells and OPC derived from newborn rat brain.