The isolation and characterization of lactoferrin from sow milk and boar seminal plasma.
The isolation and characterization of lactoferrin from sow milk and boar seminal plasma.
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母猪乳汁和公猪精浆中乳铁蛋白的分离和表征。
DOI：
10.1530/jrf.0.0420579
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
J. C. Boursnell
中科院分区：
文献类型：
--
作者：
T. Roberts;J. C. Boursnell
从母猪乳中分离的乳铁蛋白（约0-6 mg/ml）显示出色谱上均质，电泳和对单特异性抗乳铁蛋白抗血清的反应支持了这一观察结果。等电聚焦显示蛋白质的多种形式（即p，9-3至10-0）被神经氨酸酶转化为一种形式（即，9-65）。公猪精浆含有免疫学上相同的乳铁蛋白（0-1至0-5 mg/ml），其与公猪精子强烈结合。
Lactoferrin isolated from sow milk (about 0-6 mg/ml) was shown to be chromatographically homogeneous, an observation supported by electrophoresis and by reaction against monospecific anti-lactoferrin antiserum. Isoelectric focusing showed multiple forms of the protein (i.e.p., 9-3 to 10-0) converted by neuraminidase to one form (i.e.p., 9-65). Boar seminal plasma contains immunologically identical lactoferrin (0-1 to 0-5 mg/ml) which binds strongly to boar spermatozoa.