Microgravity effects on Drosophila melanogaster behavior and aging. Implications of the IML-2 experiment
Microgravity effects on Drosophila melanogaster behavior and aging. Implications of the IML-2 experiment
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DOI：
10.1016/0168-1656(96)01407-1
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发表时间：
1996-06-27
影响因子：
4.1
通讯作者：
Marco, R
中科院分区：
文献类型：
--
作者：
Benguria, A;Grande, E;Marco, R
早些时候的太空实验表明，暴露在微重力下的年轻雄性果蝇显示出衰老的加速。在14.5天的航天飞机飞行中，我们派出了300只年轻的雄性果蝇，目的是证实这些发现，并根据线粒体新陈代谢的变化可能参与衰老反应的提议，确定果蝇行为的变化是否对这种影响负责。通过反复录像，我们发现，果蝇在太空中的活动活动有了非常显著的增加。在生理活力分析(交配和负向地趋向性)和寿命曲线方面，雄鼠在恢复后表现出加速的衰老反应。微重力暴露的果蝇线粒体16S核糖体RNA比地面对照果蝇线粒体16S核糖体RNA的下降幅度更大，这也表明线粒体代谢参与其中。另一方面，与地面上暴露在类似离心机中的苍蝇相比，平行1xg离心机对照组的寿命曲线没有显示出这样的差异。雌性果蝇也增加了它们的运动活动，但在寿命曲线上没有表现出明显的变化。这些结果从目前多细胞真核生物衰老机制的角度进行了讨论。
Earlier Space experiments had indicated that young male Drosophila flies exposed to microgravity showed an acceleration in aging. In a 14.5-day Space Shuttle Flight we sent 300 young male flies with the purpose of confirming these findings and to establish whether changes in the behavior of the flies were responsible for the effect in accordance with the proposal that alterations in mitochondrial metabolism may be involved in the aging response. By repeatedly video-recording, we have found a very marked increase in the locomotor activity of the fruitflies in Space. The males showed an accelerated aging response upon recovery, both in terms of physiological vitality assays (mating and negative geotaxis) and of life-span curves. The involvement of mitochondrial metabolism is also suggested by the finding of a greater decrease in mitochondrial 16S ribosomal RNA in the microgravity exposed flies than in ground controls. On the other hand, a parallel 1 x g centrifuge control did not show such differences in the Life-span curves when compared to flies exposed to a similar centrifugation on the ground. Drosophila females also increased their locomotor activity but did not show differential changes in the life-span curves. These results are discussed in terms of the current mechanisms of aging in multicellular eukaryotic organisms.