The Bioelectric Field of the Pattern Electroretinogram in the Mouse
The Bioelectric Field of the Pattern Electroretinogram in the Mouse
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DOI：
10.1167/iovs.12-10720
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发表时间：
2012-12-01
影响因子：
4.4
通讯作者：
Porciatti, Vittorio
中科院分区：
文献类型：
--
作者：
Chou, Tsung-Han;Porciatti, Vittorio
目的。比较小鼠视网膜模式电图（PERG）与闪烁视网膜电图（FERG）的生物电场关系。32只C57BL/6J小鼠的每只眼的PERGs和FERGs记录使用角膜银环参照头后皮下针。PERG刺激为0.05周期/度的水平光栅，98%的对比度每秒反转2次。适应光的FERG刺激是明亮的频闪。刺激被呈现为单眼或双眼。部分实验单眼注射TTX，对侧眼注射生理盐水。与同侧眼相比，对侧被遮挡眼的PERG振幅略大（1.14 x, P < 0.01），潜伏期略长（+1.57 ms, P < 0.01）。双眼刺激下，PERG振幅比单眼大（1.67倍，P < 0.01）。在受刺激的眼内注射TTX可显著降低双眼的PERG。单眼ferg仅在受刺激的眼睛中可记录，TTX可适度降低。双眼和单眼ferg的振幅相似。PERG和FERG在小鼠体内产生不同的生物电场。PERG生物电场符合一个轴与眼轴正交的偶极子模型，而FERG的标准偶极子模型是同轴的。PERG生物电场的可能来源是靠近巩膜的无髓鞘视神经轴突。研究结果对青光眼和视神经疾病小鼠模型中PERG信号的产生及其改变提供了新的见解。（Invest Ophthalmol Vis Sci. 2012;53:8086-8092） DOI: 10.1167/iovs.12-10720
PURPOSE. To compare the bioelectric field associated with the pattern electroretinogram (PERG) with that of the flash electroretinogram (FERG) in the mouse.METHODS. PERGs and FERGs were recorded from each eye in 32 C57BL/6J mice using corneal silver loops referenced to a subcutaneous needle on the back of the head. PERG stimuli were horizontal gratings of 0.05 cycles per degree and 98% contrast reversing 2 times per second. Light-adapted FERG stimuli were bright strobe flashes. Stimuli were presented either monocularly or binocularly. In some experiments, TTX was injected in one eye and saline in the contralateral eye.RESULTS. The PERG recorded from the contralateral, occluded eye had slightly larger amplitude (1.14 x, P < 0.01) and longer latency (+1.57 ms, P < 0.01) compared with the ipsilateral eye. Under binocular stimulation, the PERG amplitude was much larger (1.67 x, P < 0.01) than the monocular amplitude. TTX injected in the stimulated eye drastically reduced the PERG in both eyes. Monocular FERGs were recordable from the stimulated eye only and were moderately reduced by TTX. Binocular and monocular FERGs had similar amplitudes.CONCLUSIONS. PERG and FERG generate different bioelectric fields in the mouse. The PERG bioelectric field is consistent with a dipole model whose axis is orthogonal to the eye axis, whereas the standard dipole model for the FERG is coaxial. Possible sources of the PERG bioelectric field are unmyelinated optic nerve axons adjacent to the sclera. Results provide new insights on the generators of the PERG signal and its alterations in mouse models of glaucoma and optic nerve diseases. (Invest Ophthalmol Vis Sci. 2012;53:8086-8092) DOI: 10.1167/iovs.12-10720