Age-related and sex-specific effects on architectural properties and biomechanical response of the C57BL/6N mouse femur, tibia and ulna
Age-related and sex-specific effects on architectural properties and biomechanical response of the C57BL/6N mouse femur, tibia and ulna
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DOI：
10.1016/j.bonr.2020.100266
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发表时间：
2020-06-01
期刊：
影响因子：
2.5
通讯作者：
Ganesh, Thiagarajan
中科院分区：
文献类型：
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作者：
Mumtaz, Hammad;Dallas, Mark;Ganesh, Thiagarajan
众所周知，衰老会降低骨质量和骨强度。我们试图确定衰老如何影响各种长骨的生物力学和结构特性，以及性别是否影响与年龄相关的差异/变化。虽然研究人员已经广泛研究了小鼠个体骨骼的这些变化，但还没有对同一只小鼠骨骼的变化进行全面研究，以研究随着衰老的变化。我们对股骨、胫骨和尺骨进行了三点弯曲试验和显微计算机断层扫描（microCT）分析。利用三点弯曲试验来计算生物力学参数，并且还使用高分辨率microCT进行成像以揭示皮质和小梁微结构。将C57 BL/6 N小鼠分为三个年龄组：6个月、12个月和22个月。每个年龄和性别组由6-7只小鼠组成。采用三点弯曲试验计算每块骨的极限失效载荷（UL）、弹性刚度（ES）、弹性模量（E）和绕弯曲轴的惯性矩（MOI）。分析所有骨骼的MicroCT扫描，以使用CTan的MicroCT分析确定每组织体积的皮质骨体积（C.BV/TV）、每组织体积的松质骨体积（Tb.BV/TV）、皮质骨面积（B.Ar），并测试与生物力学参数的相关性。股骨UL的平均值（标准误）从19.8（0.6）N降至12.8（1.1）N（p
Aging is known to reduce bone quality and bone strength. We sought to determine how aging affects the bio-mechanical and architectural properties of various long bones, and if sex influences age related differences/changes. While researchers have extensively studied these changes in individual bones of mice, there is no comprehensive study of the changes in the bones from the same mice to study the changes with aging. We performed three point bending tests and microcomputed tomography (microCT) analysis on femurs, tibiae and ulnae. Three point bending tests were utilized to calculate biomechanical parameters and imaging was also performed using high resolution microCT to reveal both cortical and trabecular microarchitecture C57BL/6N mice were divided into three age groups: 6, 12 and 22 months. Each age and sex group consisted of 6-7 mice. The ultimate load to failure (UL), elastic stiffness (ES), modulus of elasticity (E) and the moment of inertia about bending axis (MOI) for each bone was calculated using three point bending test. MicroCT scans of all the bones were analyzed to determine cortical bone volume per tissue volume (C.BV/TV), trabecular bone volume per tissue volume (Tb.BV/TV), cortical bone area (B.Ar) using CTAn's microCT analysis and tested for correlation with the biomechanical parameters. Mean (standard error) values of UL in femur decreased from 19.8(0.6) N to 12.8(1.1) N (p