Soluble IL-2 receptor in AIDS. Correlation of its serum level with the classification of HIV-induced diseases and its characterization.
Soluble IL-2 receptor in AIDS. Correlation of its serum level with the classification of HIV-induced diseases and its characterization.
复制标题
艾滋病中的可溶性 IL-2 受体。
作者：
M. Honda;K. Kitamura;K. Matsuda;Y. Yokota;N. Yamamoto;R. Mitsuyasu;J. Chermann;T. Tokunaga
通过使用荧光夹心ELISA法，在HIV感染血友病患者和其他HIV感染患者的血清中均检测到IL-2R水平升高。血清IL-2R水平反映了美国疾病控制中心对HIV所致疾病的分类。此外，IL-2R水平与CD_4细胞计数、淋巴细胞计数、CD_4-CD_8比值降低呈显著负相关，与白细胞计数或B细胞计数均无相关性。针对IVD感染组艾滋病患者血清IL-2R水平显著升高的情况，采用体积排阻法、高压聚焦层析和H48亲和高效液相色谱依次纯化了血清IL-2R。经H48抗体免疫沉淀和SDS-PAGE测定，IL-2R等电点分别为4.2和3.8，而MR仅为45 kDa。然而，在艾滋病患者的PBMC或19个HIV-I或HIV-II感染的细胞系中的任何一个细胞中，细胞和培养上清IL-2R的产生都没有增加。相反，成人T细胞白血病患者的PBMC和表达人类T细胞淋巴病毒-I或-II的细胞系细胞产生可溶性IL-2R。并与成人T细胞白血病患者比较，讨论了HIV感染者血清IL-2R水平升高的机制。
By using a fluorescence sandwich ELISA, elevated IL-2R levels were detected in the sera from both HIV-infected hemophiliacs and other HIV-infected patients. The serum IL-2R levels were reflective of the classification of HIV-induced diseases by the Centers for Disease Control. Moreover, the IL-2R levels were negatively correlated most prominently with CD4 cell counts, with lymphocyte counts, and with a decrease in the CD4-CD8 ratio but not with either WBC counts or B cell counts. As striking elevations of serum IL-2R were noted in AIDS patients with group IVD infection, the serum IL-2R was purified sequentially by using size-exclusion HPLC, high-pressure chromatofocusing, and H48 affinity HPLC. The isoelectric point values of IL-2R were separated into 4.2 and 3.8, whereas the Mr was determined to be only 45 kDa by immunoprecipitation with H48 antibody followed by SDS-PAGE. However, production of cellular and supernatant IL-2R was not elevated in PBMC of patients with AIDS or in any of the 19 HIV-I- or HIV-II-infected cell line cells. In contrast, PBMC from patients with adult T cell leukemia and cell line cells that expressed human T cell lymphotropic virus -I or -II produced soluble IL-2R, constitutively. The mechanisms by which serum levels of IL-2R might be elevated in HIV-infected patients are discussed in comparison with that in adult T cell leukemia patients.