A remodeling system for the oligosaccharide chains on glycoproteins with microbial endo-β-N-acetylglucosaminidases
A remodeling system for the oligosaccharide chains on glycoproteins with microbial endo-β-N-acetylglucosaminidases
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DOI：
10.1016/j.bbagen.2006.09.003
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发表时间：
2006-11-01
影响因子：
3
通讯作者：
Yamamoto, Kenji
中科院分区：
文献类型：
--
作者：
Fujita, Kiyotaka;Yamamoto, Kenji
Endo-M是来自毛霉的Endo-β-N-乙酰氨基葡萄糖苷酶，它将唾液酸肽的复合型寡糖转移到部分脱糖蛋白(N-乙酰氨基葡萄糖结合蛋白)上，该蛋白是用Endo-H，Endo-β-N-乙酰氨基葡萄糖苷酶从褶皱链霉菌的糖蛋白中去除高甘露糖型低聚糖而得到的。这一发现表明，糖蛋白上的高甘露糖型寡糖可以通过内切-M的糖基化活性转变为复合型寡糖。这是首次报道了具有不同底物特异性的微生物内切-β-N-乙酰氨基葡萄糖苷酶对糖蛋白上不同类型低聚糖的重塑体系的建立。Endo-M是从酵母菌产生的糖蛋白体外合成含有复合型寡糖的糖蛋白的有力工具。(C)2006爱思唯尔B.V.保留所有权利。
Endo-M, endo-beta-N-acetylglucosaminidase from Mucor hiemalis, transferred the complex type oligosaccharide of sialoglycopeptide to partially deglycosylated proteins (N-acetylglucosamine-attached proteins), which were prepared by excluding high-mannose type oligosaccharides from glycoproteins with Endo-H, endo-beta-N-acetylglucosaminidase from Streptomyces plicatus. This finding indicated that the high-mannose type oligosaccharides on glycoproteins can be changed to complex type ones by the transglycosylation activity of Endo-M. This is the first report of the establishment of a remodeling system for the different types of oligosaccharides on glycoproteins with microbial endo-beta-N-acetylglucosaminidases having different substrate specificities. Endo-M is a powerful tool for the in vitro synthesis of glycoproteins containing complex type oligosaccharides from glycoproteins produced by yeast. (c) 2006 Elsevier B.V. All rights reserved.