Developmental expression of mouse steroidogenic factor-1, an essential regulator of the steroid hydroxylases.
Developmental expression of mouse steroidogenic factor-1, an essential regulator of the steroid hydroxylases.
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DOI：
10.1210/mend.8.5.8058073
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发表时间：
1994-05
影响因子：
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通讯作者：
Y. Ikeda;W. Shen;H. Ingraham;K. Parker
中科院分区：
文献类型：
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作者：
Y. Ikeda;W. Shen;H. Ingraham;K. Parker
作为了解其在类固醇生成中的作用的第一步，我们研究了类固醇生成因子-1 （SF-1）的发育概况，这是一种调节类固醇羟化酶的核受体。SF-1转录本首先出现在胚胎第9天（E9）的泌尿生殖嵴中，这可能是肾上腺和性腺类固醇生成细胞的来源。到了E11，肾上腺和性腺明显分离后，整个肾上腺原基都检测到SF-1转录本。此后，肾上腺中SF-1的表达定位于皮质。与其在调节胆固醇侧链切割酶（SCC）中的作用一致，SF-1在SCC之前表达。在性腺发育的性别未分化阶段（E9-E12），所有胚胎在生殖嵴表达SF-1。随着雄性睾丸索的形成，SF-1转录本在整个睾丸中弥漫性表达，而SCC mRNA则局限于间质。这些SF-1和SCC之间的差异反映了SF-1在支持细胞中的表达，如Northern印迹和原位杂交所示。与它在胚胎睾丸中的持续表达相反，SF-1转录本在E13.5-E16.5之间从卵巢中消失，仅在妊娠晚期（E18.5）重新出现。因此，SF-1在胚胎性腺中的表达是两性二态的。再加上在支持细胞中证实了SF-1 mRNA，这些数据表明SF-1在性腺发育中发挥的作用不同于调节类固醇生成酶。此外，SF-1在胚胎前脑中表达，暗示在神经发育中起作用。
As an initial step toward understanding its role in steroidogenesis, we studied the developmental profile of steroidogenic factor-1 (SF-1), a nuclear receptor that regulates the steroid hydroxylases. SF-1 transcripts first appear on embryonic day 9 (E9) in the urogenital ridge, the probable source of steroidogenic cells of both adrenals and gonads. By E11, after the adrenals and gonads are clearly separate, SF-1 transcripts are detected throughout the adrenal primordium. Thereafter, adrenal expression of SF-1 localizes to the cortex. Consistent with its proposed role in regulating cholesterol side-chain cleavage enzyme (SCC), SF-1 is expressed before SCC. During the sexually undifferentiated stage of gonadal development (E9-E12), all embryos express SF-1 in the genital ridge. As testicular cords form in males, SF-1 transcripts are diffusely expressed throughout the testis, whereas SCC mRNA is limited to the interstitium. These differences between SF-1 and SCC reflect SF-1 expression by Sertoli cells, as shown by Northern blotting and in situ hybridization. In contrast to its persistent expression in the embryonic testis, SF-1 transcripts disappear from the ovary between E13.5-E16.5, reappearing only during late gestation (E18.5). Thus, expression of SF-1 in the embryonic gonad is sexually dimorphic. Coupled with the demonstration of SF-1 mRNA in Sertoli cells, these data suggest that SF-1 plays a role in gonadal development distinct from regulating the steroidogenic enzymes. Additionally, SF-1 is expressed in the embryonic forebrain, implying a role in neural development.