ELF4 regulates GIGANTEA chromatin access through subnuclear sequestration.
ELF4 regulates GIGANTEA chromatin access through subnuclear sequestration.
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DOI：
10.1016/j.celrep.2013.02.021
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发表时间：
2013-03-28
期刊：
影响因子：
8.8
通讯作者：
Nam HG
中科院分区：
文献类型：
--
作者：
Kim Y;Lim J;Yeom M;Kim H;Kim J;Wang L;Kim WY;Somers DE;Nam HG
包括植物在内的许多生物都使用生物钟来测量白天和黑夜的持续时间。植物昼夜节律系统中的日节律是由多个互锁的转录/翻译环以及空间调节（如核转位）产生的。GIGANTEA（GI）是拟南芥中重要的生物钟成分之一，与其他核定位的昼夜节律调节因子一样，具有独特的核体。然而，很少有人知道的动力学或GI亚核定位的作用。在这里，我们的特点GI亚核区室化，并确定在昼夜条件下意想不到的动态变化。我们进一步确定EARLY FLOWER 4（ELF 4）作为GI核分布的调节因子，通过物理相互作用。ELF 4将GI从核质中隔离至离散的核体，其中GI结合CONSTANS（CO）的启动子。我们认为ELF 4对GI的亚核区室化有助于光周期开花的调节。
Many organisms, including plants, use the circadian clock to measure the duration of day and night. Daily rhythms in the plant circadian system are generated by multiple interlocked transcriptional/translational loops and also by spatial regulations such as nuclear translocation. GIGANTEA (GI), one of the key clock components in Arabidopsis, makes distinctive nuclear bodies like other nuclear-localized circadian regulators. However, little is known about the dynamics or roles of GI subnuclear localization. Here, we characterize GI subnuclear compartmentalization and identify unexpected dynamic changes under diurnal conditions. We further identify EARLY FLOWERING 4 (ELF4) as a regulator of GI nuclear distribution through a physical interaction. ELF4 sequesters GI from the nucleoplasm, where GI binds the promoter of CONSTANS (CO), to discrete nuclear bodies. We suggest that the subnuclear compartmentalization of GI by ELF4 contributes to the regulation of photoperiodic flowering.