Anti-arthritogenic and cardioprotective action of hesperidin and daidzein in collagen-induced rheumatoid arthritis
Anti-arthritogenic and cardioprotective action of hesperidin and daidzein in collagen-induced rheumatoid arthritis
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DOI：
10.1007/s11010-016-2830-y
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发表时间：
2016-12-01
影响因子：
4.3
通讯作者：
Nafees, K. A.
中科院分区：
文献类型：
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作者：
Ahmad, Shafeeque;Alam, Khursheed;Nafees, K. A.
动脉粥样硬化与慢性炎症过程有关。脂蛋白尤其是低密度脂蛋白或其变体以及炎症标志物水平的变化是动脉粥样硬化的危险因素。在本研究中，通过在 Wistar 白化大鼠的尾部区域皮内注射胶原悬浮液，建立了类风湿性关节炎的实验模型。同时，口服橙皮苷(50mg/kg体重)和黄豆苷元(20mg/kg体重)的混悬液。这些化合物每天早上和晚上给药，持续 21 天。研究了实验大鼠膝关节匀浆中炎症标志物以及血浆脂蛋白的水平。橙皮苷和大豆苷元的施用导致关节弹性蛋白酶活性、TNF-α和丙二醛水平显着降低（p < 0.001）。此外，关节炎评分和组织学结果支持测试化合物的抗炎作用。有趣的是，测试化合物还降低了血浆低密度脂蛋白胆固醇、极低密度脂蛋白胆固醇和甘油三酯，但增加了高密度脂蛋白胆固醇的水平。因此，测试化合物改善了动脉粥样硬化的危险因素。此外，橙皮苷和大豆苷元的抗氧化作用是显而易见的，自由基负荷的减少表明用橙皮苷和大豆苷元治疗的关节炎动物血浆中总抗氧化剂水平的增加。在一项单独的体外实验中，证明了橙皮苷对 2,2-二苯基-1-三硝基苯肼和 2,2-联氮双-3-乙基苯并噻唑啉-6-磺酸具有增强的自由基清除活性。天然存在的测试化合物，特别是橙皮苷的抗炎、降血脂和抗氧化作用，似乎对类风湿性关节炎和动脉粥样硬化非常有效。因此，食用它们可能有助于预防或至少延缓易感人群这些疾病的发作。
Atherosclerosis has been linked to chronic inflammatory processes. Changes in the levels of lipoproteins, especially low-density lipoprotein or its variants, as well as inflammatory markers are risk factors for the atherosclerosis. In the present study, an experimental model of rheumatoid arthritis was developed by administrating collagen suspension intradermally in the tail region of Wistar albino rats. At the same time, a suspension of hesperidin (50 mg/kg body weight) and daidzein (20 mg/kg body weight) was orally administrated. The compounds were given in the morning and evening for 21 days. Levels of inflammatory markers in the homogenate of knee joints of experimental rats as well as plasma lipoproteins were investigated. The administration of hesperidin and daidzein caused significant (p < 0.001) decrease in articular elastase activity, TNF-alpha, and malondialdehyde levels. Further, arthritis scoring and histological findings supported the anti-inflammatory actions of the test compounds. Interestingly, the test compounds also lowered the plasma low-density lipoprotein cholesterol, very low-density lipoprotein cholesterol, and triglyceride but increased the level of high-density lipoprotein cholesterol. The test compounds thus ameliorated the risk factors of atherosclerosis. Furthermore, antioxidant roles of hesperidin as well as daidzein were evident from decrease in free radical load demonstrated as increase in total antioxidant level in plasma of arthritic animals treated with hesperidin and daidzein. In a separate in vitro experiment, enhanced free radical scavenging activity of hesperidin was demonstrated against 2,2-diphenyl-1-picrylhydrazyl and 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid. The anti-inflammatory, hypolipidemic, and antioxidant actions of the naturally occurring test compounds, particularly hesperidin, seem to be quite effective against rheumatoid arthritis and atherosclerosis. Thus, their consumption may be helpful in prevention or at least delaying the onset of these diseases in susceptible individuals.