Diversity and geochemical community assembly processes of the living rare biosphere in a sand-and-gravel aquifer ecosystem in the Midwestern United States
Diversity and geochemical community assembly processes of the living rare biosphere in a sand-and-gravel aquifer ecosystem in the Midwestern United States
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DOI：
10.1038/s41598-019-49996-z
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发表时间：
2019-09
影响因子：
4.6
通讯作者：
Kyosuke Yamamoto;K. Hackley;W. Kelly;S. Panno;Y. Sekiguchi;R. Sanford;Wen-Tso Liu;Y. Kamagata;H. Tamaki
中科院分区：
文献类型：
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作者：
Kyosuke Yamamoto;K. Hackley;W. Kelly;S. Panno;Y. Sekiguchi;R. Sanford;Wen-Tso Liu;Y. Kamagata;H. Tamaki
自然微生物群落由数量有限的丰富物种和极其多样的稀有物种组成，称为稀有生物圈。最近的研究表明，稀有生物圈不仅是一个不活跃的休眠种群，而且可能在生态系统中发挥重要的功能作用。然而，稀有生物圈的结构，活动和社区组装过程知之甚少。在这项研究中，我们评估了目前和活的微生物群落结构，包括在含水层生态系统中的稀有种群，Mahomet含水层，美国，通过16S rDNA和rRNA扩增子深度测序。13个地下水样品根据“整个”群落结构形成了三个不同的组，当关注“稀有”亚群落（<总丰度的0.1%）时，得到了相同的分组，而“丰富”亚群落（>1.0%）则给出了不同的分组。在相关性分析中，观察到的分组模式与几个地球化学因子相关，不仅整个群落的结构，而且罕见的亚群落结构与含水层生态系统中的地球化学剖面相关。我们的研究结果首先表明，生活在含水层系统中的稀有生物圈的代谢潜力，以适应当地的地球化学因素，决定了社区组装过程。
Natural microbial communities consist of a limited number of abundant species and an extraordinarily diverse population of rare species referred to as the rare biosphere. Recent studies have revealed that the rare biosphere is not merely an inactive dormant population but may play substantial functional roles in the ecosystem. However, structure, activity and community assembly processes of the rare biosphere are poorly understood. In this study, we evaluated the present and living microbial community structures including rare populations in an aquifer ecosystem, the Mahomet Aquifer, USA, by both 16S rDNA and rRNA amplicon deep sequencing. The 13 groundwater samples formed three distinct groups based on the “entire” community structure, and the same grouping was obtained when focusing on the “rare” subcommunities (<0.1% of total abundance), while the “abundant” subcommunities (>1.0%) gave a different grouping. In the correlation analyses, the observed grouping pattern is associated with several geochemical factors, and structures of not only the entire community but also the rare subcommunity are correlated with geochemical profiles in the aquifer ecosystem. Our findings first indicate that the living rare biosphere in the aquifer system has the metabolic potential to adapt to local geochemical factors which dictate the community assembly processes.