Cellular compatibility of bone-like apatite containing silicon species.
Cellular compatibility of bone-like apatite containing silicon species.
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DOI：
10.1002/jbm.a.31509
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发表时间：
2008-04
期刊：
影响因子：
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通讯作者：
A. Obata;T. Kasuga
中科院分区：
文献类型：
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作者：
A. Obata;T. Kasuga
采用溶胶-凝胶法制备了碳酸钙/聚乳酸（Si-CCPC）复合材料。将Si-CCPC浸泡在模拟体液中3天，导致在Si-CCPC上形成含硅物种的骨样羟基磷灰石层（b-HA（Si））。通过使用成骨细胞样细胞（MC3T3-E1细胞）、人间充质干细胞（MSC）和成骨细胞（HOB）的培养试验，在细胞相容性中估计b-HA（Si）。与常规b-HA（无硅物质）相比，b-HA（Si）上的MC3T3-E1细胞的细胞数和MSC的碱性磷酸酶活性显著增加。培养1天后，MC3T3-E1细胞的细胞铺展在具有和不具有硅物质的层上增强。预期b-HA（Si）促进细胞增殖和成骨分化，并且认为b-HA层对细胞铺展起重要作用。HOB培养14天和21天后，在b-HA（Si）上观察到骨结节形成。b-HA（Si）作为骨组织工程支架材料具有很大的应用潜力。
A calcium carbonate/siloxane-containing poly-(lactic acid) composite (Si-CCPC) was prepared by a sol-gel method. Si-CCPC was immersed in simulated body fluid for 3 days, resulting in the formation of bone-like hydroxyapatite layer containing silicon species (b-HA(Si)) on Si-CCPC. The b-HA(Si) was estimated in the cellular compatibility by culture tests using osteoblast-like cells (MC3T3-E1 cells), human mesenchymal stem cells (MSCs) and human osteoblasts (HOBs). Cellular number of MC3T3-E1 cells and alkaline phosphatase activity of MSCs on the b-HA(Si) increased significantly more in comparison with that on the conventional b-HA (without silicon species). Cellular spreading of MC3T3-E1 cells after 1 day of culturing was enhanced on the layer with and without silicon species. The b-HA(Si) was expected to enhance cellular proliferation and osteogenic differentiation, and the b-HA layer was believed to play the important role for cellular spreading. After 14 and 21 days of HOBs-culturing, bone nodules formation was observed on the b-HA(Si). The b-HA(Si) has great potential in use as scaffolds for bone tissue engineering.