In vitro and in vivo proposal of an artificial esophagus
In vitro and in vivo proposal of an artificial esophagus
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DOI：
10.1002/jbm.a.30666
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发表时间：
2006-06-15
影响因子：
4.9
通讯作者：
Parnigotto, Pier Paolo
中科院分区：
文献类型：
--
作者：
Marzaro, Maurizio;Vigolo, Simonetta;Parnigotto, Pier Paolo
人工材料和自体组织用于食管重建，在长期随访中常引起狭窄和渗漏等并发症。本研究旨在探讨在体外构建由同种脱细胞食管基质和自体平滑肌细胞组成的组织工程食管的可行性。通过洗涤剂-酶法获得的无细胞基质不存在任何主要组织相容性复合物标记物，并表达bFGF作为蛋白，在鸡胚绒毛尿囊膜（CAM）上显示体内血管生成活性。此外，它们支持细胞粘附，并且因为在接种后24小时，支架似乎完全被SMC覆盖。为了验证我们的结构的生物相容性，在猪食管壁中产生的缺损使用含有和不含有自体SMC培养物的同源脱细胞基质覆盖。术后3周，仅由脱细胞基质组成的补片显示出更严重的炎症反应，并且α-平滑肌肌动蛋白免疫染色呈阴性。相比之下，细胞基质植入物表现出SMC的向内生长，显示出早期组织成小束。总的来说，这些结果表明，修补组成的同源食管脱细胞基质和自体平滑肌细胞可能代表一个有前途的组织工程方法修复食管损伤。(c)2006 Wiley Periodicals，Inc.
Artificial materials and autologous tissues used for esophageal reconstruction often induce complications like stenosis and leakage at long-term follow-up. This study evaluates the possibility to obtain in vitro an implantable tissue-engineered esophagus composed of homologous esophageal acellular matrix and autologous smooth muscle cells (SMCs). Acellular matrices obtained by detergent-enzymatic method did not present any major histocompatibility complex marker and expressed bFGF as protein, showing angiogenic activity in vivo on the chick embryo chorioallantoic membrane (CAM). Moreover, they supported cell adhesion, and inasmuch as just after 24 h from seeding, the scaffold appeared completely covered by SMCs. To verify the biocompatibility of our constructs, defects created in the porcine esophageal wall were covered using homologous acellular matrices with and without Cultures of autologous SMCs. At 3 week from surgery, the patches composed of only acellular matrices showed a more severe inflammatory response and were negative for alpha-smooth muscle actin immunostaining. In contrast, the cell-matrix implants presented ingrowth of SMCs, showing an early organization into small fascicules. Collectively, these results suggest that patches composed of homologous esophageal acellular matrix and autologous SMCs may represent a promising tissue-engineering approach for the repair of esophageal injuries. (c) 2006 Wiley Periodicals, Inc.