A test on different aspects of diatom processing techniques
A test on different aspects of diatom processing techniques
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DOI：
10.1007/s10811-007-9292-2
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发表时间：
2008-08
影响因子：
3.3
通讯作者：
S. Blanco;I. Alvarez;C. Cejudo
中科院分区：
文献类型：
--
作者：
S. Blanco;I. Alvarez;C. Cejudo
为了评估硅藻加工技术中的四个处理步骤（添加到样品中的过氧化氢的量、消化反应的时间、离心的使用以及将硅藻壳沉降到盖玻片上所使用的方法）对所得显微载玻片质量的不同方面的影响进行了析因实验：有机和无机物质的浓度，载玻片中硅藻壳分布的密度、随机性和均匀性，以及破碎的硅藻壳和硅藻壳的丰度。腰围视图。结果表明，任何实验处理对样品中破碎硅藻壳的比例没有显着影响。在载玻片质量评估的任何方面，氧化反应的持续时间都没有显着影响。对于 6 小时的氧化反应，样品：过氧化氢的比例为 1:1，可用于优化方案。尽管更耗时，但使用连续倾析代替离心更适合获得更好的结果。通过将盖玻片在室温下干燥以改善硅藻壳的分布，也可以提高载玻片质量。
A factorial experiment was carried out in order to assess the effects of four treatment steps in diatom processing techniques (the quantity of hydrogen peroxide added to the sample, the time allowed to the digestion reaction, the use of centrifugation and the method used to settle the frustules onto the coverslip) on different aspects of the quality of the resulting microscopic slide: the concentration of organic and inorganic matter, the density, randomness and homogeneity of the frustule distribution in the slide, and the abundance of broken frustules and girdle views. The results show no significant effects of any of the experimental treatments on the proportion of broken frustules in the samples. No significant effects were related to the duration of the oxidation reaction in any of the aspects evaluated in the quality of the slides. A sample:hydrogen peroxide ratio of 1:1 for a 6-h oxidizing reaction can be used to optimize the protocol. Despite being more time consuming, the use of successive decantations instead of centrifuging is more appropriate to achieve better results. Slide quality can also be improved by leaving the coverslips to dry at room temperature in order to improve the distribution of frustules.