HPLC analysis of phenolic antioxidants, tocopherols and triglycerides
HPLC analysis of phenolic antioxidants, tocopherols and triglycerides
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酚类抗氧化剂、生育酚和甘油三酯的 HPLC 分析
DOI：
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
C. Taeschler
中科院分区：
文献类型：
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作者：
N. Andrikopoulos;H. Brueschweiler;H. Felber;C. Taeschler
采用反相C18柱，水/乙腈/甲醇/异丙醇梯度洗脱，高效液相色谱法分离甘油三酯、9种合成酚类抗氧化剂、天然抗氧化剂生育酚和α-生育酚乙酸酯。除了用异丙醇/己烷稀释油之外，不需要进一步的实施例制备。采用紫外检测。合成的抗氧化剂丙基十二烷基没食子酸酯、辛基十二烷基没食子酸酯、十二烷基没食子酸酯、3-叔丁基-4-羟基茴香醚、叔丁基对苯二酚、3，5-二叔丁基-4-羟基甲苯、2，6-二叔丁基-4-羟甲基苯酚、2，4，5-三羟基丁酰苯酮和去甲二氢愈创木酸以及α-和δ-生育酚和α-生育酚乙酸酯基线分离;而β-和γ-生育酚一起洗脱。在λ = 215 nm处检测到的甘油三酯根据分配数进行分离。在λ = 215 nm处的吸收显示饱和和不饱和甘油三酯。在λ = 280 nm处的吸收表明甘油三酯具有共轭不饱和度，这与油的精炼和热处理有关。氧化不饱和甘油三酯在γ = 230 nm处显示吸收。蓖麻油酸（一种羟基单饱和酸）的甘油三酯具有相同的紫外光谱。同时检测抗氧化剂和甘油三酯可用于研究油中抗氧化剂的抑制作用。
Triglycerides, together with nine synthetic phenolic antioxidants most commonly used to prevent oxidation of edible oils and fats, as well as the natural antioxidants tocopherols andα-tocopherol acetate, were separated by high performance liquid chromatography by means of a reversed phase C18-column and gradient elution with water/acetonitrile/methanol/isopropanol. Besides dilution of the oil with isopropanol/hexane, no further example preparation was required. UV detection was applied. The synthetic antioxidants propyldodecylgallate, octyldodecylgallate, dodecylgallate, 3-tert-butyl-4-hydroxyanisol,tert-butylhydroquinone, 3,5-di-terf-butyl-4-hydroxytoluene, 2,6-di-tert-butyl-4-hydroxymethyl-phenol, 2,4,5-trihydroxybutyrophenone and nordihydro-quaiaretic acid, as well asα- andδ-tocopherol andα-tocopherol acetate were base-line separated;β- andγ-tocopherol, however, eluted together. The triglycerides, detected at λ = 215 nm, were separated according to then-partition number. The absorption at λ = 215 nm revealed saturated and unsaturated triglycerides. The absorption at λ = 280 nm indicated triglycerides with conjugated unsaturation, relating information about refining and heat treatment of the oil. Oxidized unsaturated triglycerides showed absorption at γ = 230 nm. Triglycerides of ricinoleic acid, a hydroxymonoun-saturated acid, gave identical UV spectra. The simultaneous detection of antioxidants and triglycerides may be used to study inhibition effects by antioxidants in oils.