Investigation of deltamethrin resistance in salmon lice (Lepeophtheirus salmonis) provides no evidence for roles of mutations in voltage-gated sodium channels.
Investigation of deltamethrin resistance in salmon lice (Lepeophtheirus salmonis) provides no evidence for roles of mutations in voltage-gated sodium channels.
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DOI：
10.1002/ps.6120
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发表时间：
2020-10
影响因子：
4.1
通讯作者：
Claudia Tschesche;Michaël Bekaert;David I. Bassett;Chris Mitchell;B. North;Sally Boyd;G. Carmona-Antoñanzas-G.-C
中科院分区：
文献类型：
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作者：
Claudia Tschesche;Michaël Bekaert;David I. Bassett;Chris Mitchell;B. North;Sally Boyd;G. Carmona-Antoñanzas-G.-C
背景拟除虫菊酯溴氰菊酯用于治疗养殖鲑鱼受寄生鲑鱼虱Lepeophtheirus salmonis（Krøyer）侵染。然而，溴氰菊酯对鲑鱼灭虱的功效受到抗性发展的威胁。在陆生节肢动物中，电压门控钠通道（Nav）（拟除虫菊酯的分子靶标）的敲低抗性（kdr）突变可导致溴氰菊酯抗性。一个L.鲑鱼钠通道同源物（LsNav 1.3 I936 V）。同时，对溴氰菊酯抗性也有不同程度的提高。鲑鱼已被证明是母系遗传的，并与线粒体DNA（mtDNA）突变有关。本研究评估了上述假定的kdr突变作为实验室菌株和田间种群溴氰菊酯抗性决定因素的潜在作用。鲑鱼结果溴氰菊酯抗性L.鲑属菌株IoA-02表达LsNav 1.3 I936 V突变，但对非酯拟除虫菊酯醚菊酯敏感，如果抗性由Nav突变引起，则拟除虫菊酯抗性节肢动物通常对该化合物交叉抗性。在来自IoA-02雄性和缺乏kdr突变的药物敏感雌性之间的杂交的家族中，溴氰菊酯抗性与LsNav 1.3基因座的基因型无关（p>0.05）。同样，在苏格兰的野外种群中，L. LsNav 1.3 I936 V与溴氰菊酯抗性无相关性。而A14013 G和A9030 G基因型与溴氰菊酯抗性显著相关（p<0.001）。结论在所研究的L.结果表明，对溴氰菊酯的抗性与LsNav 1.3 I936 V突变无关，而与线粒体DNA突变密切相关。
BACKGROUND The pyrethroid deltamethrin is used to treat infestations of farmed salmon by parasitic salmon lice, Lepeophtheirus salmonis (Krøyer). However, the efficacy of deltamethrin for salmon delousing is threatened by resistance development. In terrestrial arthropods, knockdown resistance (kdr) mutations of the voltage-gated sodium channel (Nav ), the molecular target for pyrethroids, can cause deltamethrin resistance. A putative kdr mutation of an L. salmonis sodium channel homologue (LsNav 1.3 I936V) has previously been identified. At the same time, deltamethrin resistance of L. salmonis has been shown to be inherited maternally and to be associated with mitochondrial DNA (mtDNA) mutations. The present study assessed potential roles of the above putative kdr mutation as a determinant of deltamethrin resistance in laboratory strains and field populations of L. salmonis. RESULTS The deltamethrin resistant L. salmonis strain IoA-02 expresses the LsNav 1.3 I936V mutation but was susceptible to the non-ester pyrethroid etofenprox, a compound against which pyrethroid resistant arthropods are usually cross-resistant if resistance is caused by Nav mutations. In a family derived from a cross between an IoA-02 male and a drug-susceptible female lacking the kdr mutation, deltamethrin resistance was not associated with the genotype at the LsNav 1.3 locus (p>0.05). Similarly, in Scottish field populations of L. salmonis, LsNav 1.3 I936V showed no association with deltamethrin resistance. In contrast, genotypes at the mtDNA loci A14013G and A9030G were significantly associated with deltamethrin resistance (p<0.001). CONCLUSION In the studied L. salmonis isolates, deltamethrin resistance was unrelated to the LsNav 1.3 I936V mutation, but showed close association with mtDNA mutations.