A 3-D microelectrode system for handling and caging single cells and particles
A 3-D microelectrode system for handling and caging single cells and particles
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DOI：
10.1016/s0956-5663(99)00006-8
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发表时间：
1999-03-15
影响因子：
12.6
通讯作者：
Fuhr, G
中科院分区：
文献类型：
--
作者：
Müller, T;Gradl, G;Fuhr, G
我们已经设计并构建了几种设计的3-D微电极系统组成的两层电极结构分开的40 μ m厚的聚合物间隔形成一个流动通道。电极元件如漏斗、对准器、笼和开关由交流（AC）和/或旋转电场驱动。它们被设计为使用负介电泳（nDEP）聚焦、捕获和分离直径为10-30 μ m的真核细胞（Jurkat）或乳胶颗粒。电极宽度近似于10 μ m，并且活性电极表面已经被最小化以减少溶液的加热。通过与多达三个发生器和/或实验室制造的配电箱一起工作，实现了不同电极元件的更灵活的使用。漏斗、校准器或开关的电极在5-11 V、5-15 MHz下操作，可以在高达3500 μ m/s的流速下实现颗粒的有效处理。细胞可以在PBS中以高达300 μ m/s的流速有效地排列。胶乳颗粒可以保留在介电保持笼（DFC）或校准器内，对抗40-200 μ m/s的层流，使用8 V的振幅，5-15 MHz。细胞可以以高达50 μ m/s的流速保存。数值计算的介电力和诱导膜电位举行笼中给出了不同的电导率在不同的应用频率的解决方案。(C)1999年Elsevier Science S.A. All rights reserved.
We have designed and constructed several designs of 3-D microelectrode systems consisting of two layers of electrode structures separated by a 40 mu m thick polymer spacer forming a flow channel. The electrode elements such as funnel, aligner, cage and switch are driven by alternating current (AC) and/or rotating electric fields. They are designed to focus, trap and separate eukaryotic cells (Jurkat) or latex particles with a diameter of 10-30 mu m using negative dielectrophoresis (nDEP). The electrode width is similar to 10 mu m and active electrode surfaces have been minimised in order to reduce heating of the solution. A more flexible employment of the different electrode elements was realised by working with up to three generators, and/or a laboratory-made distribution box. The electrodes of the funnel, aligner or switch were operated with 5-11 V at 5-15 MHz, efficient handling of particles could be achieved with flow rates up to 3500 mu m/s. Cells could be aligned effectively at flow rates up to 300 mu m/s in PBS. Latex particles could be retained within the dielectric held cages (DFC) or aligner against a laminar flow of 40-200 mu m/s using an amplitude of 8 V at 5-15 MHz. Cells could be held at flow rates up to 50 mu m/s. Numerical calculations for dielectric forces and the induced membrane potential in held cages are given for solutions of different conductivities at different applied frequencies. (C) 1999 Elsevier Science S.A. All rights reserved.