UPSTREAM ACTIVATION SEQUENCE-DEPENDENT ALTERATION OF CHROMATIN STRUCTURE AND TRANSCRIPTION ACTIVATION OF THE YEAST GAL1-GAL10 GENES
UPSTREAM ACTIVATION SEQUENCE-DEPENDENT ALTERATION OF CHROMATIN STRUCTURE AND TRANSCRIPTION ACTIVATION OF THE YEAST GAL1-GAL10 GENES
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DOI：
10.1128/mcb.9.4.1721
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发表时间：
1989-04-01
影响因子：
5.3
通讯作者：
KORNBERG, RD
中科院分区：
文献类型：
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作者：
FEDOR, MJ;KORNBERG, RD
被抑制的GAL1-GAL10启动子区域的定位核小体阵列特征转化为诱导状态的更容易接近的构象，被发现依赖于上游激活序列、转录的正调节因子GAL4蛋白和诱导剂半乳糖。GAL4蛋白上游活化序列复合物对相邻染色质结构的影响不需要其他启动子序列。尽管受抑制状态组蛋白保护的序列在转录诱导后更容易被微球菌核酸酶和（甲氧基丙基edta）铁（II）切割，但含有这些序列的dna -蛋白质颗粒保留了核小体的电泳迁移性，这表明启动子区域在转录激活条件下可能与核小体相关。
Conversion of the positioned nucleosome array characteristic of the repressed GAL1-GAL10 promoter region to the more accessible conformation of the induced state was found to depend on the upstream activation sequence, GAL4 protein, a positive regulator of transcription, and galactose, the inducing agent. The effect of the GAL4 protein-upstream activation sequence complex on the structure of adjacent chromatin required no other promoter sequences. Although sequences protected by histones in the repressed state became more accessible to micrococcal nuclease and (methidiumpropyl-EDTA)iron(II) cleavage following induction of transcription, DNA-protein particles containing these sequences retained the electrophoretic mobility of nucleosomes, indicating that the promoter region can be associated with nucleosomes under conditions of transcription activation.