Acetylcholine innervation of sensory and motor neocortical areas in adult cat: A choline acetyltransferase immunohistochemical study
Acetylcholine innervation of sensory and motor neocortical areas in adult cat: A choline acetyltransferase immunohistochemical study
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DOI：
10.1016/0891-0618(96)00132-9
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发表时间：
1996-08-01
影响因子：
2.8
通讯作者：
Descarries, L
中科院分区：
文献类型：
--
作者：
Avendano, C;Umbriaco, D;Descarries, L
应用胆碱乙酰转移酶（ChAT）免疫细胞化学方法，观察了成年猫初级运动区（4 γ）和初级感觉区（3a、3b、41和17）的乙酰胆碱神经支配分布。这些轴突通常很薄，有无数不同大小的血管曲张。还可见一些较厚和较光滑的纤维，偶尔可见异常大的静脉曲张簇。总体而言，17区的神经支配密度低于其他区域。在各区域，I层均为致密神经支配。在区域17中，下层的神经支配是相当均匀的，但在其他区域中是图案化的。在区域4伽马和3a，层II，上III和V表现出优先神经支配。IV层的神经支配在3b区和41区最强。3a区是4 γ和3b之间的过渡区。除17区外，乙酰胆碱酯酶染色的层状模式与ChAT一致。根据目前的数据，这种皮质神经支配在不同物种的分布，其假定的超微结构特征，它似乎可能是这样的区域和层状功能对着广泛的，调节作用的乙酰胆碱。
Light microscopic choline acetyltransferase (ChAT) immunocytochemistry was used to examine the distribution of the acetylcholine innervation in primary motor (4 gamma) and sensory (3a, 3b, 41 and 17) cortical areas of adult cat. In every area, scattered immunoreactive cell bodies were present and a relatively dense meshwork of ChAT immunoreactive axons pervaded the whole cortical thickness. These axons were generally thin and bore innumerable varicosities of different sizes. A few thicker and smoother fibers and occasional clusters of unusually large varicosities were also visible. Overall, area 17 was less densely innervated than the other areas. In each area, layer I showed the densest innervation. Innervation of underlying layers was rather uniform in area 17, but patterned in other areas. In areas 4 gamma and 3a, layers II, upper III and V showed preferential innervation. Innervation of layer IV was the strongest in areas 3b and 41. Area 3a was transitional between 4 gamma and 3b. Except in area 17, the laminar pattern of acetylcholinesterase staining was consistent with that of ChAT. In the light of current data on the distribution of this cortical innervation in different species, and of its presumed ultrastructural features, it appears likely that such regional and laminar features subtend widespread, modulatory roles of ACh.