Inactivation of a wheat protein kinase gene confers broad-spectrum resistance to rust fungi
Inactivation of a wheat protein kinase gene confers broad-spectrum resistance to rust fungi
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DOI：
10.1016/j.cell.2022.06.027
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发表时间：
2022-08-05
期刊：
影响因子：
64.5
通讯作者：
Wang, Xiaojie
中科院分区：
文献类型：
--
作者：
Wang, Ning;Tang, Chunlei;Wang, Xiaojie
小麦作物经常受到由条锈菌引起的大流行性条锈病的破坏。 sp。小麦（Pst）。在这里，我们鉴定并表征了一种小麦受体样细胞质激酶基因 TaPsIPK1，它赋予了对该病原体的易感性。 PsSpg1 是一种对 Pst 毒力至关重要的分泌型真菌效应子，可以结合 TaPsIPK1，增强其激酶活性，并促进其核定位，在核定位中磷酸化转录因子 TaCBF1d 以进行基因调控。 TaCBF1d 的磷酸化会改变下游基因的转录活性。在两年的田间试验中，小麦中 TaPsIPK1 的 CRISPR-Cas9 失活赋予了 Pst 广谱抗性，且不影响重要的农艺性状。 TaPsIPK1 的破坏会导致免疫启动，但不会持续激活防御反应。总而言之，TaPsIPK1 是一种已知的锈病效应子靶标的易感基因，并且具有通过基因改造开发持久抗锈病能力的巨大潜力。
Wheat crops are frequently devastated by pandemic stripe rust caused by Puccinia striiformis f. sp. tritici (Pst). Here, we identify and characterize a wheat receptor-like cytoplasmic kinase gene, TaPsIPK1, that con-fers susceptibility to this pathogen. PsSpg1, a secreted fungal effector vital for Pst virulence, can bind TaPsIPK1, enhance its kinase activity, and promote its nuclear localization, where it phosphorylates the tran-scription factor TaCBF1d for gene regulation. The phosphorylation of TaCBF1d switches its transcriptional activity on the downstream genes. CRISPR-Cas9 inactivation of TaPsIPK1 in wheat confers broad-spectrum resistance against Pst without impacting important agronomic traits in two years of field tests. The disruption of TaPsIPK1 leads to immune priming without constitutive activation of defense responses. Taken together, TaPsIPK1 is a susceptibility gene known to be targeted by rust effectors, and it has great potential for devel-oping durable resistance against rust by genetic modifications.