HETEROLOGOUS EXPRESSION OF ACTIVE MANGANESE PEROXIDASE FROM PHANEROCHAETE-CHRYSOSPORIUM USING THE BACULOVIRUS EXPRESSION SYSTEM
HETEROLOGOUS EXPRESSION OF ACTIVE MANGANESE PEROXIDASE FROM PHANEROCHAETE-CHRYSOSPORIUM USING THE BACULOVIRUS EXPRESSION SYSTEM
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DOI：
10.1016/0006-291x(91)91903-p
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发表时间：
1991-09-16
影响因子：
3.1
通讯作者：
TIEN, M
中科院分区：
文献类型：
--
作者：
PEASE, EA;AUST, SD;TIEN, M
The cDNA encoding Mn peroxidase isozyme H4 fromPhanerochaete chrysosporiumwas recombined into a baculovirus and heterologously expressed in Sf9 cells. The recombinant Mn peroxidase has the same molecular weight as the native enzyme as determined by SDS-PAGE and cross-reacts with a Mn peroxidase-specific antibody. The recombinant enzyme has a slightly lower pI than the native fungal isozyme H4 indicating some differences in post-translational modification. Phenol red, guaiacol, and vanillylacetone, substrates of the native Mn peroxidase, are oxidized by the recombinant enzyme. All of the activities are dependent on both Mn (II) and H2O2.
