A LysM Receptor Heteromer Mediates Perception of Arbuscular Mycorrhizal Symbiotic Signal in Rice
A LysM Receptor Heteromer Mediates Perception of Arbuscular Mycorrhizal Symbiotic Signal in Rice
复制标题
LysM 受体异聚物介导水稻丛枝菌根共生信号的感知
DOI：
10.1016/j.molp.2019.10.015
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发表时间：
2019-12-02
期刊：
影响因子：
27.5
通讯作者：
Wang, Ertao
中科院分区：
文献类型：
--
作者：
He, Jiangman;Zhang, Chi;Wang, Ertao
共生微生物改善植物对养分的吸收。为了启动与丛枝菌根（AM）真菌的互利共生，植物感知AM真菌分泌的Myc因子，包括脂壳低聚糖（LCOs）和短链壳低聚糖（CO 4/CO 5）。然而，Myc因子感知的分子机制仍然难以捉摸。在这项研究中，我们确定了一个异源聚体的LysM受体样激酶组成的OsMYR 1/OsLYK 2和OsCERK 1介导的AM真菌在水稻的感知。CO 4直接与OsMYR 1结合，促进该受体复合物的二聚化和磷酸化。与对照相比，Osmyr 1和Oscerk 1突变体水稻植株对Myc因子的敏感性降低，AM定殖率降低。我们通过表达嵌合受体来工程化转基因水稻，所述嵌合受体分别用来自蒺藜苜蓿的同源结瘤因子受体MtNFP和MtLYK 3的那些替换OsMYR 1和OsCERK 1的胞外域。转基因植株表现出增加钙振荡响应结瘤因素相比，对照水稻。我们的研究为水稻AM共生信号的感知提供了重要的机制见解。嵌合Nod/Myc受体的表达实现了产生宿主固氮细菌的谷物的潜在重要步骤。
Symbiotic microorganisms improve nutrient uptake by plants. To initiate mutualistic symbiosis with arbuscular mycorrhizal (AM) fungi, plants perceive Myc factors, including lipochitooligosaccharides (LCOs) and short-chain chitooligosaccharides (CO4/CO5), secreted by AM fungi. However, the molecular mechanism of Myc factor perception remains elusive. In this study, we identified a heteromer of LysM receptor-like kinases consisting of OsMYR1/OsLYK2 and OsCERK1 that mediates the perception of AM fungi in rice. CO4 directly binds to OsMYR1, promoting the dimerization and phosphorylation of this receptor complex. Compared with control plants, Osmyr1 and Oscerk1 mutant rice plants are less sensitive to Myc factors and show decreased AM colonization. We engineered transgenic rice by expressing chimeric receptors that respectively replaced the ectodomains of OsMYR1 and OsCERK1 with those from the homologous Nod factor receptors MtNFP and MtLYK3 of Medicago truncatula. Transgenic plants displayed increased calcium oscillations in response to Nod factors compared with control rice. Our study provides significant mechanistic insights into AM symbiotic signal perception in rice. Expression of chimeric Nod/Myc receptors achieves a potentially important step toward generating cereals that host nitrogen-fixing bacteria.