New molecular data on mammalian Hepatozoon species (Apicomplexa: Adeleorina) from Brazil and Spain
New molecular data on mammalian Hepatozoon species (Apicomplexa: Adeleorina) from Brazil and Spain
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DOI：
10.1645/ge-464r.1
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发表时间：
2006-02-01
影响因子：
1.3
通讯作者：
Barba-Carretero, JC
中科院分区：
文献类型：
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作者：
Criado-Fornelio, A;Ruas, JL;Barba-Carretero, JC
利用分子生物学技术研究了从巴西的13只狐狸和15只负鼠中分离出的肝体动物物种之间的系统发育关系，以及从西班牙的15只木屐、20只狐狸、45只啮齿动物和330只家猫中分离出的肝体动物物种之间的系统发育关系。通过扩增18S rRNA基因和随后的测序证实了血红蛋白感染。负鼠中未发现血红蛋白感染。犬科动物中肝体虫的患病率范围为26.6%（有症状的家犬）至90%（西班牙狐狸）。四个不同的H。检测犬的基因型，以及H.与美国菌株有97%的同源性。两只西班牙猫被一种肝虫寄生（0.6%流行率），与H.狗对西班牙啮齿类动物进行的DNA扩增分析显示，2个库票（Glareolus）被一种肝虫物种感染（患病率4.44%），与猫肝虫物种具有95%的序列同一性。系统发育分析表明，肝虫属是一个单系属，其中来自肉食性哺乳动物的种类（前猫肝虫属、前猫肝虫属H. americanum和H.犬科动物（canis）似乎是低等脊椎动物和啮齿动物的姐妹谱系。这种关联表明H.美洲蜱在蜱和食肉动物（犬科动物或猫科动物，或两者）中进化，而不是在其他体外寄生虫和其他类型的哺乳动物中进化。
Molecular techniques were used to examine the phylogenetic relationships among Hepatozoon species isolated from 13 foxes and 15 opossums from Brazil, and from 15 clogs, 20 foxes, 45 rodents, and 330 domestic cats from Spain. Hemogregarine infection was confirmed by amplification of the 18S rRNA gene and later sequencing. No hemogregarine infections were found in opossums. The prevalence of Hepatozoon in canids ranged from 26.6% (symptomatic domestic dogs) to 90% (Spanish foxes). Four different H. canis genotypes were detected, as well as an H. americanum-related protozoan (97% identical to the USA strain). Two Spanish cats were parasitized by a Hepatozoon species (0.6% prevalence) that showed 96% sequence identity to H. canis. DNA amplification assays performed oil Spanish rodents showed 2 bank votes (Clethrionomys glareolus) to be infected by a Hepatozoon species (4.44% prevalence) with 95% sequence identify to Hepatozoon sp. from cats. Phylogenetic analysis showed Hepatozoon to be a monophyletic genus, in which species from carnivorous mammals (Hepatozoon sp. front cats, H. americanum and H. canis) appear as a sister lineage of that of lower vertebrates and rodents. This association suggests that H. americanum evolved in ticks and carnivores (either canids, or felids, or both) rather than in other ectoparasites and other types of mammal.