EFFECT OF HORMONES AND GROWTH-FACTORS ON ALKALINE-PHOSPHATASE ACTIVITY AND COLLAGEN-SYNTHESIS IN CULTURED RAT CALVARIAE
EFFECT OF HORMONES AND GROWTH-FACTORS ON ALKALINE-PHOSPHATASE ACTIVITY AND COLLAGEN-SYNTHESIS IN CULTURED RAT CALVARIAE
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DOI：
10.1016/0026-0495(83)90149-x
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发表时间：
1983-01-01
影响因子：
9.8
通讯作者：
CANALIS, E
中科院分区：
文献类型：
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作者：
CANALIS, E
关于激素和生长因子对碱性磷酸酶的直接影响的研究仅限于甲状旁腺激素（PTH）和1,25-二羟基维生素D3 [1,25（OH）2D3]，并且与骨形成的其他参数相比尚未相比。胰岛素，PTH，1,25（OH）2d3，表皮和成纤维细胞生长因子（EGF，FGF）是否对碱性磷酸酶活性和I型的影响进行了检查，[.Alpha.1（i）] 2.Alpha.2，2.Alpha.2，2，Alpha.2， 21天的胎儿大鼠钙钙培养物中的胶原蛋白合成。治疗24小时和96小时后，胰岛素增加，而PTH，1,25（OH）2D3，EGF和FGF抑制钙化钙化钙碱磷酸酶活性，以及将3H-Pro掺入胶原酶消化蛋白和I型胶原中。测试的药物不会影响碱性磷酸酶释放到培养基中。尽管I型胶原蛋白是唯一检测到的胶原蛋白，但也可能合成了少量的另一个胶原蛋白。 96小时后，激素对碱性磷酸酶活性和I型胶原蛋白合成的影响比连续暴露于测试的试剂的24小时后的幅度高，并且2个参数在96 h和r = 0.97后与0.88相关（r = 0.88）治疗）。胰岛素增加钙碱性磷酸酶的活性和I型胶原蛋白合成中的钙蛋白酶，而PTH为1,25（OH）2d3，EGF和FGF具有抑制作用。这些药物显然会影响成骨细胞功能。
Studies on the direct effects of hormones and growth factors on bone alkaline phosphatases have been limited to parathyroid hormone (PTH) and 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] and have not been compared to other parameters of bone formation. Insulin, PTH, 1,25(OH)2D3, epidermal and fibroblast growth factors (EGF, FGF) were examined for their effects on alkaline phosphatase activity and type I, [.alpha.1(I)]2.alpha.2, collagen synthesis in cultures of 21 day fetal rat calvariae. After 24 h and 96 h of treatment, insulin increased whereas PTH, 1,25(OH)2D3, EGF and FGF inhibitd calvarial alkaline phosphatase activity and the incorporation of 3H-Pro into collagenase-digestible protein and type I collagen. The agents tested did not affect the release of alkaline phosphatase into the culture medium. Although type I collagen was the only collagen detected, a small amount of another collagen might have been also synthesized. The hormonal effects on alkaline phosphatase activity and type I collagen synthesis were of greater magnitude after 96 h than after 24 h of continuous exposure to the agents tested and the 2 parameters correlated well (r = 0.88 after 96 h and r = 0.97 after 24 h of treatment). Insulin increases bone alkaline phosphatase activity and type I collagen synthesis in calvariae whereas PTH, 1,25(OH)2D3, EGF and FGF have an inhibitory effect. These agents apparently affect osteoblastic function.