DETERMINATION OF PESTICIDE METABOLITES IN HUMAN URINE USING AN ISOTOPE-DILUTION TECHNIQUE AND TANDEM MASS-SPECTROMETRY
DETERMINATION OF PESTICIDE METABOLITES IN HUMAN URINE USING AN ISOTOPE-DILUTION TECHNIQUE AND TANDEM MASS-SPECTROMETRY
复制标题
DOI：
10.1093/jat/19.5.323
复制
发表时间：
1995-09-01
影响因子：
2.5
通讯作者：
NEEDHAM, LL
中科院分区：
文献类型：
--
作者：
HILL, RH;SHEALY, DB;NEEDHAM, LL
A method that measures 12 analytes in urine and reflects possible exposure to pesticides was developed. The sample preparation involves enzyme hydrolysis and solvent extraction through the use of laboratory robotics, followed by phase-transfer catalysis derivatization and silica cleanup. Samples are analyzed by capillary gas chromatography and tandem mass spectrometry using an isotope dilution technique with13C-labeled internal standards. The limit of detection is 1 µg/L (1 part per billion) for most analytes, and most analytes have a linear response up to 100 µg/L. The precision of the method is reflected in the variation observed in quality control materials over 33 months; the variation averaged 17% for these analytes. On the basis of the detectable analyte levels of unspiked urine samples collected from unexposed volunteers, this method can be used to measure the low levels necessary for establishing reference range values of the selected pesticides or metabolites.
