SVEP1 expression is regulated in estrogen-dependent manner
SVEP1 expression is regulated in estrogen-dependent manner
复制标题
DOI：
10.1002/jcp.20895
复制
发表时间：
2007-03-01
影响因子：
5.6
通讯作者：
Benayahu, D.
中科院分区：
文献类型：
--
作者：
Shur, I.;Zemer-Tov, E.;Benayahu, D.
SVEP1蛋白包括与选择素超家族相关的模块和细胞表面分子中的其他基序。早些时候，我们在体内和体外证明了SVEP1在成骨细胞中的表达；在本研究中，我们详细阐述了17β-雌二醇对SVEP1的调节。SVEP1基因在假手术大鼠体内有表达，但在去卵巢大鼠体内不表达。我们证明了17β-E2处理增加了成骨细胞中SVEP1的表达水平。在乳腺癌(BC)细胞中也发现了SVEP1，已知的BC细胞在从原发肿瘤转移时驻留在骨中。免疫组织化学和荧光激活细胞分选(FACS)检测不同BC细胞系中SVEP1的表达。染色质免疫沉淀(ChIP)分析雌激素受体(ER)与可能的SVEP1启动子的结合。我们证明了17βE_2或ICI 182,780处理会影响这种结合，并调节BC细胞中SVEP1的mRNA和蛋白水平。我们认为，SVEP1可以作为一种有用的生物标志物，用于研究雌激素影响的局部微环境中细胞相互作用的机制。
The SVEP1 protein comprises modules related to the selectin super family and other motifs found in cell surface molecules. Earlier, we demonstrated that SVEP1 is expressed in osteogenic cells both in vivo and in vitro; in the current study we elaborate on the regulation of SVEP1 by 17 beta-Estradiol (17 beta E2). SVEP1 message is expressed in vivo by bone marrow cells of sham-operated rats, but not in estrogen-depleted ovariectomized (OVX) rats. We demonstrated that 17 beta E2 treatment increases the level of the SVEP1 expression in cultured osteoblasts. SVEP1 was identified also in breast carcinoma (BC) cells known to reside in bone when metastasized from the primary tumor. SVEP1 expression was demonstrated by immunohistochemistry and fluorescence-activated cell sorting (FACS) on various BC cell lines. The chromatin immunoprecipitation (ChIP) assay was applied to analyze the estrogen receptor (ER) binding to the putative SVEP1 promoter. We demonstrated that treatment with 17 beta E2 or ICI 182,780 affects this binding and regulates the mRNA and protein levels of SVEP1 in BC cells. We propose that SVEP1 may serve as a useful biomarker for studying the mechanism of cells interactions within the local microenvironment affected by estrogen.