The genomic organization of the human corticotropin-releasing factor type-1 receptor
The genomic organization of the human corticotropin-releasing factor type-1 receptor
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DOI：
10.1016/s0378-1119(98)00322-9
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发表时间：
1998-09-28
期刊：
影响因子：
3.5
通讯作者：
Suda, T
中科院分区：
文献类型：
--
作者：
Sakai, K;Yamada, M;Suda, T
我们确定了人类CRF 1型受体（hCRF-R1）的基因组结构。编码hCRF-R1的基因由至少14个外显子组成，跨度超过20千碱基。hCRF-R1的三种已报道的异构体通过选择性剪接来源于相同的基因。第一个hCRF-R1以最高亲和力结合CRF并转导对CRF应答最敏感的cAMP积累，其编码在总共13个外显子中，唯一排除的是外显子6。第二同种型含有额外的29个氨基酸序列，其对应于外显子6。与第一种亚型不同，第三种亚型缺少40个氨基酸的序列，对应于外显子3。外显子-内含子边界与共有序列的边界相同。内含子在编码序列中的位置与人CRF-R1、大鼠CRF-R1、人CRF-R2 α和属于人胰高血糖素受体家族的其他基因相似。(C)1998 Elsevier Science B. V.保留所有权利。
We determined the genomic organization of human CRF type-1 receptor (hCRF-R1). The gene coding for hCRF-R1 consists of at least 14 exons and spans over 20 kilobases. hCRF-R1's three reported isoforms originate from the same gene by alternative splicing. The first hCRF-R1, which binds to CRF with the highest affinity and transduces the most sensitive cAMP accumulation in response to CRF, is encoded in a total of 13 exons, the only one excluded being exon 6. The second isoform contains an additional 29-amino acid sequence which corresponds to exon 6. Unlike the first isoform, the third lacks a 40-amino acid sequence, corresponding to exon 3. Exon-intron boundaries are the same as that of the consensus sequence. Locations of introns in the coding sequence are similar to human CRF-R1, rat CRF-R1, human CRF-R2 alpha and others belonging to the human glucagon receptor family. (C) 1998 Elsevier Science B.V. All rights reserved.