Chromatin-Dependent Repression of the Arabidopsis Floral Integrator Genes Involves Plant Specific PHD-Containing Proteins
Chromatin-Dependent Repression of the Arabidopsis Floral Integrator Genes Involves Plant Specific PHD-Containing Proteins
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DOI：
10.1105/tpc.114.130781
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发表时间：
2014-10-01
期刊：
影响因子：
11.6
通讯作者：
Pineiro, Manuel
中科院分区：
文献类型：
--
作者：
Lopez-Gonzalez, Leticia;Mouriz, Alfonso;Pineiro, Manuel
组蛋白修饰之间的相互作用调节发育中主调控基因的表达。染色质效应蛋白结合组蛋白修饰并将表观遗传状态翻译成控制发育的基因表达模式。在这里，我们表明，两个拟南芥旁系同源编码植物特异性蛋白与植物同源结构域（PHD）基序，短寿命（SHL）和早螺栓在短日子（EBS），功能在染色质介导的抑制花的起始和发挥独立的作用，在控制基因调控开花。先前的结果表明，花整合基因FLOWERYLOCUS T（FT）的阻遏需要EBS。我们建立SHL是必要的负调控表达的CO1（SOC1），另一个花的整合。SHL和EBS分别识别赖氨酸4处的二甲基化和三甲基化组蛋白H3，并结合SOC 1和FT的调控区。这些PHD蛋白通过阻止高水平的H3乙酰化，结合组蛋白去乙酰化酶6，在SOC 1和FT中保持无活性的染色质构象，并在调节开花时间中发挥核心作用。SHL和EBS在植物中广泛保守，但在其他真核生物中不存在，这表明由这些蛋白质介导的调控模块可能代表植物中基因表达控制的独特机制。
The interplay among histone modifications modulates the expression of master regulatory genes in development. Chromatin effector proteins bind histone modifications and translate the epigenetic status into gene expression patterns that control development. Here, we show that two Arabidopsis thaliana paralogs encoding plant-specific proteins with a plant homeodomain (PHD) motif, SHORT LIFE (SHL) and EARLY BOLTING IN SHORT DAYS (EBS), function in the chromatin-mediated repression of floral initiation and play independent roles in the control of genes regulating flowering. Previous results showed that repression of the floral integrator FLOWERING LOCUS T (FT) requires EBS. We establish that SHL is necessary to negatively regulate the expression of SUPPRESSOR OF OVEREXPRESSION OF CO1 (SOC1), another floral integrator. SHL and EBS recognize di-and trimethylated histone H3 at lysine 4 and bind regulatory regions of SOC1 and FT, respectively. These PHD proteins maintain an inactive chromatin conformation in SOC1 and FT by preventing high levels of H3 acetylation, bind HISTONE DEACETYLASE6, and play a central role in regulating flowering time. SHL and EBS are widely conserved in plants but are absent in other eukaryotes, suggesting that the regulatory module mediated by these proteins could represent a distinct mechanism for gene expression control in plants.