Circadian CLOCK gene polymorphisms in relation to sleep patterns and obesity in African Americans: findings from the Jackson heart study.
Circadian CLOCK gene polymorphisms in relation to sleep patterns and obesity in African Americans: findings from the Jackson heart study.
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DOI：
10.1186/s12863-017-0522-6
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发表时间：
2017-06-23
期刊：
影响因子：
2.9
通讯作者：
Davis SK
中科院分区：
文献类型：
--
作者：
Riestra P;Gebreab SY;Xu R;Khan RJ;Gaye A;Correa A;Min N;Sims M;Davis SK
昼夜节律调节关键的生物过程，而内在生物钟机制的失调影响睡眠模式和肥胖症的发生。CLOCK（昼夜运动输出周期蛋白kaput）基因编码影响多种代谢途径（包括葡萄糖和脂质稳态）的分子昼夜节律钟的核心转录因子。本研究的主要目的是评估CLOCK单核苷酸多态性（SNPs）与体重指数（BMI）之间的关联。我们还在来自杰克逊心脏研究的2962名参与者（1116名男性和1810名女性）中评估了SNPs与BMI相关因素（如睡眠时间和质量、脂联素和瘦素）的关联。所选23个CLOCK基因SNPS的基因型数据通过使用IMPUTE 2软件和来自1000基因组计划的参考相位数据进行插补获得。我们发现CLOCK SNP rs 2070062与睡眠时间之间存在显著相关性，在调整欧洲血统（PEA），社会经济地位（SES），体重指数（BMI），饮酒和吸烟状况经多重检验校正后达到显著性阈值。此外，我们发现CLOCK SNP rs6853192与较长的睡眠时间以及rs6820823、rs3792603和rs 11726609与BMI存在名义相关性。然而，这些关联在多次检验校正后未达到显著性阈值。在这项工作中，CLOCK基因变异与睡眠时间和BMI相关，这表明这些多态性对昼夜节律的影响可能会影响非裔美国人的睡眠时间和体重调节。本文的在线版本（doi：10.1186/s12863-017-0522-6）包含补充材料，可供授权用户使用。
Circadian rhythms regulate key biological processes and the dysregulation of the intrinsic clock mechanism affects sleep patterns and obesity onset. The CLOCK (circadian locomotor output cycles protein kaput) gene encodes a core transcription factor of the molecular circadian clock influencing diverse metabolic pathways, including glucose and lipid homeostasis. The primary objective of this study was to evaluate the associations between CLOCK single nucleotide polymorphisms (SNPs) and body mass index (BMI). We also evaluated the association of SNPs with BMI related factors such as sleep duration and quality, adiponectin and leptin, in 2962 participants (1116 men and 1810 women) from the Jackson Heart Study. Genotype data for the selected 23 CLOCK gene SNPS was obtained by imputation with IMPUTE2 software and reference phase data from the 1000 genome project. Genetic analyses were conducted with PLINK We found a significant association between the CLOCK SNP rs2070062 and sleep duration, participants carriers of the T allele showed significantly shorter sleep duration compared to non-carriers after the adjustment for individual proportions of European ancestry (PEA), socio economic status (SES), body mass index (BMI), alcohol consumption and smoking status that reach the significance threshold after multiple testing correction. In addition, we found nominal associations of the CLOCK SNP rs6853192 with longer sleep duration and the rs6820823, rs3792603 and rs11726609 with BMI. However, these associations did not reach the significance threshold after correction for multiple testing. In this work, CLOCK gene variants were associated with sleep duration and BMI suggesting that the effects of these polymorphisms on circadian rhythmicity may affect sleep duration and body weight regulation in Africans Americans. The online version of this article (doi:10.1186/s12863-017-0522-6) contains supplementary material, which is available to authorized users.